Home > LILACS - Literatura Latinoamericana y del Caribe de Ciencias de la Salud > Search result

Results: 2

Development of a novel vector for cloning and expressing extremely toxic genes in Escherichia coli

Li, Hedan; Hao, Chengwei; Xu, Daqing.
Electron. j. biotechnol; 30 (), 2017
Background: Escherichia coli has been widely used as a host to clone and express heterologous genes. However, there are few vectors available for cloning and expressing extremely toxic genes, which limits further basic and applied research on extremely toxic proteins. Results: In this study, a novel vect...
Escherichia coli/genética, Proteínas de Escherichia coli/toxicidad, Vectores Genéticos, Triptófano/metabolismo, Regiones Promotoras Genéticas, Proteínas Co-Represoras, Genes Bacterianos, ARN sin Sentido, Isopropil Tiogalactósido/metabolismo, Reacción en Cadena de la Polimerasa, Electroforesis en Gel de Poliacrilamida, Desoxirribonucleasa BamHI/metabolismo, Western Blotting, Clonación Molecular
Fulltext

A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide

Clement, Herlinda; Flores, Vianey; Diego-Garcia, Elia; Corrales-Garcia, Ligia; Villegas, Elba; Corzo, Gerardo.
J. venom. anim. toxins incl. trop. dis; 21 (), 2015
Background:The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were co...
Arañas, Neurotoxinas, Isopropil Tiogalactósido, Isoformas de Proteínas, Reacción en Cadena de la Polimerasa, Técnicas In Vitro
Fulltext