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Experimental carcinogenesis with 7, 12-dimethylbenz(a)anthrazene (DMBA) and its inhibition with isothi-ocyanates

Morales-Herrero, María; Ortega-Medina, Isicio.
J. oral res. (Impresa); 11 (4), 2022
Introduction: DMBA is a chemical carcinogen that induces carcinomas within a few weeks of its application. We developed an experimental model of carcinogenesis induced by DMBA dissolved in 0,5% paraffin oil (DMBA-PO), verifying the inhibitory effect of the carcinogenicity of phenyl isothiocyanate (PhITC)...
9,10-Dimetil-1,2-benzantraceno/toxicidad, Anticarcinógenos/uso terapéutico, Carcinoma de Células Escamosas de Cabeza y Cuello, Isotiocianatos, Carcinogénesis, Carcinógenos, Modelos Animales
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Application of lectins for detection of glycomic differences in the epididymal duct in water buffalo (Bubalus bubalis) and dromedary camel (Camelus dromedarius)

Alkafafy, Mohamed E.
Int. j. morphol; 40 (3), 2022
SUMMARY: The present study was conducted to detect the differences in glycohistochemical features in the epididymal duct of the dromedary camel and the water buffalo. Epididymal sections (caput, corpus and cauda) from both species were stained with fluorescein isothiocyanate (FITC) conjugated lectins. Bi...
Búfalos, Camelus, Epidídimo/metabolismo, Lectinas/metabolismo, Colorantes, Isotiocianatos, Fluoresceína, Epidídimo/citología, Inmunohistoquímica
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Benzyl isothiocyanate inhibits invasion and induces apoptosis via reducing S100A4 expression and increases PUMA expression in oral squamous cell carcinoma cells

Ma, Lei; Chen, Yongjun; Han, Rui; Wang, Shuangyi.
Braz. j. med. biol. res; 52 (4), 2019
Benzyl isothiocyanate (BITC) has been shown to inhibit invasion and induce apoptosis of various types of cancer. However, its role on human oral squamous cell carcinoma (OSCC) cells is still not well elucidated. In the present study, we investigated the effect of BITC on apoptosis and invasion of SCC9 ce...
Apoptosis/efectos de los fármacos, Carcinoma de Células Escamosas/patología, Línea Celular Tumoral, Movimiento Celular/efectos de los fármacos, Proliferación Celular/efectos de los fármacos, Supervivencia Celular/efectos de los fármacos, Inmunohistoquímica, Isotiocianatos/farmacología, Ratones Desnudos, Proteína de Unión al Calcio S100A4/efectos de los fármacos, Proteína de Unión al Calcio S100A4/metabolismo
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Analysis of a suppressive subtractive hybridization library of Alternaria alternata resistant to 2-propenyl isothiocyanate

García-Coronado, Heriberto; Troncoso-Rojas, Rosalba; Tiznado-Hernández, Martín Ernesto; de la Cruz Otero, María del Carmen; Díaz-Camacho, Sylvia Páz; Báez-Flores, María Elena.
Electron. j. biotechnol; 18 (4), 2015
Background Isothiocyanates (ITCs) are natural products obtained from plants of the Brassicas family. They represent an environmentally friendly alternative for the control of phytopathogenic fungi. However, as it has been observed with synthetic fungicides, the possibility of inducing ITC-resistant strai...
Alternaria/metabolismo, Alternaria/genética, Farmacorresistencia Fúngica, Isotiocianatos, Fungicidas Industriales, Hibridación Genética, Biología Computacional, Técnicas de Hibridación Sustractiva
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Studies on statistical optimization of sulforaphane production from broccoli seed

Wu, Yuanfeng; Mao, Jianwei; Mei, Lehe; Liu, Shiwang.
Electron. j. biotechnol; 16 (6), 2013
Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major fact...
Semillas/metabolismo, Brassica, Sulfóxidos/metabolismo, Isotiocianatos/metabolismo, 28599, Cromatografía Líquida de Alta Presión, Sulfóxidos/análisis, Isotiocianatos/análisis
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Conjugação e validação de controle isotípico IgG1-FITC para uso em citometria de fluxo
Conjugation and validation of IgG1-FITC isotype control to be used in flow cytometry

Golim, Márjorie A; Deffune, Elenice; Rossi-Ferreira, Rosana; Oliveira, Ana Paula E; Padovani, Carlos Roberto; Machado, Paulo Eduardo A.
Rev. bras. hematol. hemoter; 29 (4), 2007
Em meados da década de 50 iniciou-se o desenvolvimento da citometria de fluxo, tecnologia que permite verificar características físico-químicas de células ou partículas suspensas em meio fluido. Esta tecnologia utiliza anticorpos monoclonais marcados com fluorocromos como ferramenta de investigaçÃ...
Isotiocianatos, Citometría de Flujo, Anticuerpos Monoclonales, Servicio de Hemoterapia, Sangre, Inmunoglobulina G, Inmunoglobulinas, Indicadores y Reactivos, Fluoresceína-5-Isotiocianato, Electroforesis en Gel de Poliacrilamida, Electroforesis, Antígenos, Anticuerpos, Isotipos de Inmunoglobulinas
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Cuantificación de sulforafano procedente de glucorafanina en retoños de brassica oleracea var. italica por cromatografía líquida de alta resolución

Navas Nájera, Rosa Patricia.
Se determino la presencia de 4.76 mg/g (0.476 % P/P) de Sulforafano de la muestra de retoños de brócoli que se cultivó bajo un sistema controlado (Tabla No. 3), temperatura 18-22 °C, pH 6.5, fotoperíodo de 11-13 horas de luz, humedad relativa intermedia a baja, además el brócoli fue cortado al 5º...
Brassica/química, Isotiocianatos, Plantas Medicinales, Brotes de la Planta, Cromatografía
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