BACKGROUND Oxidative stress is responsible for generating DNA lesions and the 8-oxoguanine (8-oxoG) is the most commonly lesion found in DNA damage. When this base is incorporated during DNA replication, it could generate double-strand DNA breaks and cellular death. MutT enzyme hydrolyzes the 8-oxoG fro...
Antimonio/toxicidad,
Antiprotozoarios/farmacología,
Proteínas de Escherichia coli/genética,
Proteínas de Escherichia coli/metabolismo,
Escherichia coli,
Guanina/análogos & derivados,
Guanina/farmacología,
Peróxido de Hidrógeno/toxicidad,
Leishmania braziliensis/efectos de los fármacos,
Leishmania braziliensis/enzimología,
Leishmania infantum/efectos de los fármacos,
Leishmania infantum/enzimología,
Estrés Oxidativo/efectos de los fármacos,
Estrés Oxidativo/fisiología,
Pirofosfatasas/genética,
Pirofosfatasas/metabolismo,
Conejos,
Superóxido Dismutasa/genética,
Superóxido Dismutasa/metabolismo
BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected wit...
Antimonio/farmacología,
Antiprotozoarios/farmacología,
Resistencia a Medicamentos,
Citometría de Flujo,
Inmunidad Innata,
Leishmania braziliensis/efectos de los fármacos,
Leishmania braziliensis/inmunología,
Leishmania infantum/efectos de los fármacos,
Leishmania infantum/inmunología,
Monocitos/inmunología,
Monocitos/parasitología,
Óxido Nítrico/biosíntesis,
Fagocitosis/inmunología
BACKGROUND In addition to the limited therapeutic arsenal and the side effects of antileishmanial agents, drug resistance hinders disease control. In Brazil, Leishmania braziliensis causes atypical (AT) tegumentary leishmaniasis lesions, frequently refractory to treatment. OBJECTIVES The main goal of ...
Abstract In Brazil, meglumine antimoniate is the first drug of choice for mucosal leishmaniasis treatment followed by amphotericin B and pentamidine isethionate. We report the case of a patient with severe mucosal lesions caused by Leishmania (Viannia) braziliensis that were difficult to treat. Over a 14...
Cota, Betania Barros;
Tunes, Luiza Guimarães;
Maia, Daniela Nabak Bueno;
Ramos, Jonas Pereira;
Oliveira, Djalma Menezes de;
Kohlhoff, Markus;
Alves, Tânia Maria de Almeida;
Souza-Fagundes, Elaine Maria;
Campos, Fernanda Fraga;
Zani, Carlos Leomar.
BACKGROUND In a screen of extracts from plants and fungi to detect antileishmanial activity, we found that the ethyl acetate extract of the fungus Nectria pseudotrichia, isolated from the tree Caesalpinia echinata (Brazilwood), is a promising source of bioactive compounds. OBJECTIVES The aims of this st...
Ascorbate peroxidase (APX) is a redox enzyme of the trypanothione pathway that converts hydrogen peroxide (H2O2) into water molecules. In the present study, the APX gene was overexpressed in Leishmania braziliensis to investigate its contribution to the trivalent antimony (SbIII)-resistance phenotype. We...
Antimonio/farmacología,
Antiprotozoarios/farmacología,
Ascorbato Peroxidasas/metabolismo,
Western Blotting,
Resistencia a Medicamentos,
Regulación Enzimológica de la Expresión Génica,
Leishmania braziliensis/efectos de los fármacos,
Leishmania braziliensis/enzimología,
Estrés Oxidativo,
Pruebas de Sensibilidad Parasitaria,
Fenotipo,
Proteínas Protozoarias/metabolismo
ESTEVAM, ELISÂNGELA B B;
DEUS, ISABELLA P B DE;
SILVA, VANESSA P DA;
SILVA, ELIZABETH A J DA;
ALVES, CASSIA C F;
ALVES, JOSÉ MILTON;
CAZAL, CRISTIANE M;
MAGALHÃES, LIZANDRA G;
PAGOTTI, MARIANA C;
ESPERANDIM, VIVIANE R;
SOUZA, ALEX F;
MIRANDA, MAYKER L D.
ABSTRACT Leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. Since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. Thus, the search for new, more...
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania...
Meneguetti, Dionatas Ulises de Oliveira;
Lima, Renato Abreu;
Hurtado, Fernanda Bay;
Passarini, Guilherme Matos;
Macedo, Sharon Rose Aragão;
Barros, Neuza Biguinati de;
Oliveira, Flávio Augusto de Souza;
Medeiros, Patrícia Soares de Maria de;
Militão, Júlio Sancho Linhares Teixeira;
Nicolete, Roberto;
Facundo, Valdir Alves.
Abstract INTRODUCTION Maytenus guianensis is a member of the Celastraceae family that is used in traditional medicine, particularly for its anti-parasitic and anti-cancer effects. To explore the ethnopharmacological potential of this plant, the present study was designed to screen the in vitro antileish...
