Abstract INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESULT...
Abstract INTRODUCTION: This study evaluated the epidemiology of American cutaneous leishmaniasis in the immediate region of Ji-Paraná, Rondônia State. METHODS: Samples and epidemiological data were collected from 105 patients. RESULTS: Leishmania infection was observed in 58 (55.2%) patients, and ...
Abstract INTRODUCTION: In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RN...
Côrtes, Luzia Monteiro de Castro;
de Pita-Pereira, Daniela;
Farani, Priscila Silva Grijó;
Pereira, Bernardo Acácio Santini;
Dias-Lopes, Geovane;
da Silva, Franklin Souza;
Corrêa, Paloma Resende;
Silva, Roger Magno Macedo;
Côrte-Real, Suzana;
Bello, Felio Jesus;
Mendonça-Lima, Leila;
Moreira, Otacilio da Cruz;
Waghabi, Mariana Caldas;
Alves, Carlos Roberto.
BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. M...
BACKGROUND The leishmaniases are complex neglected diseases caused by protozoan parasites of the genus Leishmania. Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis in the New World. In recent studies, genomic changes such as chromosome and gene copy number variations (CNV...
Abstract Blood samples and swabs from ocular conjunctiva and mouth were obtained from 64 cats. Of 64 serum samples, 19 were positive for Leishmania antibodies by ELISA (29.80%). Eight cats were positive by PCR (12.5%) in swab samples from mouth and/or ocular mucosa. Poor kappa agreement between serologic...
Anticuerpos Antiprotozoarios/sangre,
Enfermedades de los Gatos/diagnóstico,
Enfermedades de los Gatos/parasitología,
ADN Protozoario/análisis,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania braziliensis/genética,
Leishmania braziliensis/inmunología,
Leishmania braziliensis/aislamiento & purificación,
Leishmania infantum/genética,
Leishmania infantum/inmunología,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis/diagnóstico,
Leishmaniasis/parasitología,
Filogenia,
Reacción en Cadena de la Polimerasa/veterinaria
Abstract Introduction: Mucosal leishmaniasis has a progressive course and can cause deformity and even mutilation in the affected areas. It is endemic in the American continent and it is mainly caused by Leishmania (Viannia) braziliensis. Objective: To describe a series of mucosal leishmaniasis cases a...
Leishmania braziliensis/aislamiento & purificación,
Leishmania guyanensis/aislamiento & purificación,
Leishmaniasis Mucocutánea/parasitología,
Colombia/epidemiología,
ADN Protozoario/genética,
Genes Protozoarios,
Proteínas HSP70 de Choque Térmico/genética,
Leishmania braziliensis/clasificación,
Leishmania braziliensis/genética,
Leishmania guyanensis/clasificación,
Leishmania guyanensis/genética,
Leishmaniasis Mucocutánea/complicaciones,
Leishmaniasis Mucocutánea/epidemiología,
Leishmaniasis Mucocutánea/patología,
Reacción en Cadena de la Polimerasa,
Polimorfismo de Longitud del Fragmento de Restricción,
Proteínas Protozoarias/genética,
Análisis de Secuencia de ADN,
Piel/parasitología,
Especificidad de la Especie
BACKGROUND In addition to the limited therapeutic arsenal and the side effects of antileishmanial agents, drug resistance hinders disease control. In Brazil, Leishmania braziliensis causes atypical (AT) tegumentary leishmaniasis lesions, frequently refractory to treatment. OBJECTIVES The main goal of ...
BACKGROUND Calpains are proteins belonging to the multi-gene family of calcium-dependent cysteine peptidases that undergo tight on/off regulation, and uncontrolled proteolysis of calpains is associated with severe human pathologies. Calpain orthologues are expanded and diversified in the trypanosomatids...
Western Blotting,
Calpaína/efectos de los fármacos,
Calpaína/genética,
Calpaína/metabolismo,
Calpaína/ultraestructura,
Inhibidores de Cisteína Proteinasa/farmacología,
Dipéptidos/farmacología,
Citometría de Flujo,
Regulación de la Expresión Génica,
Genoma de Protozoos/genética,
Inmunohistoquímica,
Leishmania braziliensis/química,
Leishmania braziliensis/genética,
Leishmania braziliensis/metabolismo,
Leishmania braziliensis/ultraestructura,
Macrófagos Peritoneales/metabolismo,
Ratones Endogámicos BALB C,
Microscopía Electrónica de Transmisión,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Factores de Virulencia
Leishmania braziliensis is the etiological agent of American mucosal leishmaniasis, one of the most severe clinical forms of leishmaniasis. Here, we report the assembly of the L. braziliensis (M2904) genome into 35 continuous chromosomes. Also, the annotation of 8395 genes is provided. The public availab...
