Leishmania infantum chagasi is the causative agent and Lutzomyia longipalpis is the main vector of visceral leishmaniasis in the Americas. We investigated the expression of Leishmania genes within L. longipalpis after artificial infection. mRNAs from genes involved in sugar and amino acid metabolism were...
Brasil,
Expresión Génica,
Insectos Vectores/parasitología,
Leishmania/aislamiento & purificación,
Leishmania infantum/genética,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/parasitología,
Leishmaniasis Visceral/transmisión,
Estadios del Ciclo de Vida,
Psychodidae/genética,
Psychodidae/parasitología,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos
Abstract The aim of this study was to investigate the level of exposure to Leishmania infection in stray dogs in an area of intense visceral leishmaniasis transmission in the state of Pernambuco, Brazil. Blood samples from 178 dogs were analyzed using serological and molecular assays: rapid immunochromat...
Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the ...
Sevá, Anaiá da Paixão;
Brandão, Ana Pérola Drulla;
Godoy, Silvia Neri;
Soares, Rodrigo Martins;
Langoni, Helio;
Rodrigues, Bruna Cristine;
Gava, Mariana Zanchetta e;
Zanotto, Paula Ferraz de Camargo;
Jimenez-Villegas, Tatiana;
Hiramoto, Roberto;
Ferreira, Fernando.
Abstract INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid tes...
Anticuerpos Antiprotozoarios,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/genética,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Patología Molecular,
Estudios Seroepidemiológicos
Abstract Visceral leishmaniasis is a parasitic zoonosis that mainly affects poorest and most vulnerable populations, and domestic dogs are considered to be the main source of infection to the vector and therefore humans. However, several studies have investigated the role of other vertebrate hosts in the...
Abstract The main clinical, anatomopathological, and molecular aspects of the infection by Leishmania infantum are described in two cats with multicentric cutaneous, nodular, and ulcerated lesions. The animals were submitted to a clinical examination, followed by serological, molecular and parasitologica...
Anticuerpos Antiprotozoarios/sangre,
Brasil,
Enfermedades de los Gatos/diagnóstico,
ADN de Cinetoplasto/genética,
Eutanasia Animal,
Leishmania infantum/genética,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/transmisión,
Leishmaniasis Visceral/veterinaria,
Macrófagos/parasitología,
Reacción en Cadena de la Polimerasa/veterinaria
Abstract Leishmania infantum infection in cats has been reported in several countries, including Brazil. However, the transmission of visceral leishmaniasis (VL) from cats to another host has not been proven yet. Therefore, the aim of this study was to verify the possibility of L. infantum transmission f...
Brasil,
Enfermedades de los Gatos/parasitología,
Enfermedades de los Gatos/transmisión,
ADN Protozoario/química,
Enfermedades de los Perros/parasitología,
Enfermedades de los Perros/transmisión,
Insectos Vectores/parasitología,
Leishmania infantum/genética,
Leishmaniasis Visceral/transmisión,
Leishmaniasis Visceral/veterinaria,
Psychodidae/parasitología
Abstract INTRODUCTION: Leishmania infantum was considered to be absent from Amapá until 2017 when canine infection was detected. However, there is a lack of knowledge about which reservoir species are involved in transmission in this region. METHODS: Between 2014 and 2016, 86 samples from wild mammal...
Makino, Herica;
Moreira, Janaina Marcela Assunção Rosa;
Bezerra, Kalinne Stephanie;
Otsubo, Amanda Atsumy Funakawa;
Bortolini, Juliano;
Sousa, Valéria Régia Franco;
Dutra, Valeria;
Colodel, Edson Moleta;
Nakazato, Luciano;
Almeida, Arleana do Bom Parto Ferreira de.
Abstract Leishmania infantum is a trypanosomatid that causes parasitic dermatopathy in dogs. Trypanosoma caninum is another trypanosomatid, which infects the skin of dogs, although cutaneous abnormalities are absent. This study aimed to investigate the occurrence of T. caninum infection and its associate...
Brasil/epidemiología,
Coinfección,
ADN Protozoario/genética,
Enfermedades de los Perros/epidemiología,
Enfermedades de los Perros/patología,
Leishmania infantum/genética,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/patología,
Leishmaniasis Visceral/veterinaria,
Reacción en Cadena de la Polimerasa,
Prevalencia,
Trypanosoma/genética,
Tripanosomiasis/epidemiología,
Tripanosomiasis/patología,
Tripanosomiasis/veterinaria
Dias, Renata Cristina Ferreira;
Pasquali, Aline Kuhn Sbruzzi;
Thomaz-Soccol, Vanete;
Pozzolo, Eliane Maria;
Chiyo, Luciana;
Alban, Silvana Maria;
Fendrich, Ricardo Cancio;
Almeida, Rômulo Augusto Andrade de;
Ferreira, Fernanda Pinto;
Caldart, Eloiza Teles;
Freire, Roberta Lemos;
Mitsuka-Breganó, Regina;
Bisetto Júnior, Alceu;
Navarro, Italmar Teodorico.
Abstract The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Iguaçu. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 bu...
Brasil/epidemiología,
ADN Protozoario/genética,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Enfermedades de los Perros/parasitología,
Leishmania infantum/genética,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria
