Fujimori, Mahyumi;
de Almeida, Arleana do Bom Parto Ferreira;
Barrouin-Melo, Stella Maria;
Cortez, Luiz Ricardo Paes de Barros;
Duthie, Malcolm Scott;
Hiramoto, Roberto Mitsuyoshi;
de Pinho, Flaviane Alves;
Reed, Steven Gregory;
Sousa, Valéria Régia Franco;
Souza, Nazaré Fonseca;
Soares, Rodrigo Martins;
Tolezano, José Eduardo;
Sanchez, Maria Carmen Arroyo;
Goto, Hiro.
BACKGROUND Dogs are the main peridomiciliary reservoir of Leishmania infantum thus the correct diagnosis of infection is essential for the control of the transmission and treatment as well. However, the diagnosis is based on serological assays that are not fully effective. OBJECTIVE We aimed to establ...
Antígenos de Protozoos/biosíntesis,
Anticuerpos Antiprotozoarios/sangre,
Brasil,
Enfermedades de los Perros/diagnóstico,
Ensayo de Inmunoadsorción Enzimática/métodos,
Ensayo de Inmunoadsorción Enzimática/normas,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/inmunología,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis Visceral/sangre,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/veterinaria,
Proteínas Recombinantes/inmunología,
Sensibilidad y Especificidad,
Pruebas Serológicas
Abstract Canine leishmaniasis (CanL) is a disease caused by Leishmania infantum. Serological methods are the most common diagnostic techniques used for the diagnosis of the CanL. The objective of our study was to estimate the sensitivity and specificity of one in-house ELISA kit (ELISA UNIZAR) and three ...
Anticuerpos Antiprotozoarios/sangre,
Teorema de Bayes,
Enfermedades de los Perros/diagnóstico,
Ensayo de Inmunoadsorción Enzimática/normas,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Análisis de Clases Latentes,
Leishmania infantum/inmunología,
Leishmaniasis/diagnóstico,
Leishmaniasis/veterinaria,
Pruebas Serológicas/normas,
Pruebas Serológicas/veterinaria
Abstract INTRODUCTION: Canine visceral leishmaniasis (CVL) is a public health problem, and its prevalence is associated with the coexistence of vectors and reservoirs. CVL is a protozoonosis caused by Leishmania infantum that is endemic in the southeast region of Brazil. Thus, vector and canine reservo...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Enfermedades Endémicas,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/inmunología,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Prevalencia,
Estudios Seroepidemiológicos,
Análisis Espacial
BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected wit...
Antimonio/farmacología,
Antiprotozoarios/farmacología,
Resistencia a Medicamentos,
Citometría de Flujo,
Inmunidad Innata,
Leishmania braziliensis/efectos de los fármacos,
Leishmania braziliensis/inmunología,
Leishmania infantum/efectos de los fármacos,
Leishmania infantum/inmunología,
Monocitos/inmunología,
Monocitos/parasitología,
Óxido Nítrico/biosíntesis,
Fagocitosis/inmunología
Abstract This study aimed to determine the seroprevalence, factors associated with seropositivity to Leishmania infection in dogs and spatial analysis in six municipalities in the semiarid region of Pernambuco, Brazil. Blood samples were collected from 462 dogs, 77 in each municipality, and used for sero...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/inmunología,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Prevalencia,
Factores de Riesgo,
Estudios Seroepidemiológicos,
Análisis Espacial
Abstract Blood samples and swabs from ocular conjunctiva and mouth were obtained from 64 cats. Of 64 serum samples, 19 were positive for Leishmania antibodies by ELISA (29.80%). Eight cats were positive by PCR (12.5%) in swab samples from mouth and/or ocular mucosa. Poor kappa agreement between serologic...
Anticuerpos Antiprotozoarios/sangre,
Enfermedades de los Gatos/diagnóstico,
Enfermedades de los Gatos/parasitología,
ADN Protozoario/análisis,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania braziliensis/genética,
Leishmania braziliensis/inmunología,
Leishmania braziliensis/aislamiento & purificación,
Leishmania infantum/genética,
Leishmania infantum/inmunología,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis/diagnóstico,
Leishmaniasis/parasitología,
Filogenia,
Reacción en Cadena de la Polimerasa/veterinaria
Abstract Toxoplasma gondii and Leishmania infantum are zoonotic protozoal parasites. Serum samples were obtained from 186 donkeys (Equus africanus asinus) from Portugal and assessed for antibodies to T. gondii by the modified agglutination test (MAT). For titration of antibodies to Leishmania spp. the di...
Pruebas de Aglutinación,
Anticuerpos Antiprotozoarios/sangre,
Equidae/parasitología,
Leishmania infantum/inmunología,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Portugal/epidemiología,
Estudios Seroepidemiológicos,
Toxoplasma/inmunología,
Toxoplasmosis Animal/diagnóstico,
Toxoplasmosis Animal/epidemiología
Santos, Anna Raquel Ribeiro dos;
Serufo, Ângela Vieira;
Figueiredo, Maria Marta;
Godoi, Lara Carvalho;
Vitório, Jéssica Gardone;
Marcelino, Andreza Pain;
Avelar, Daniel Moreira de;
Rodrigues, Fernandes Tenório Gomes;
Machado-Coelho, George Luiz Lins;
Medeiros, Fernanda Alvarenga Cardoso;
Jerônimo, Selma Maria Bezerra;
Oliveira, Edward José de;
Nascimento, Frederico Crepaldi;
Teixeira, Santuza Maria Ribeiro;
Gazzinelli, Ricardo Tostes;
Nagem, Ronaldo Alves Pinto;
Fernandes, Ana Paula.
BACKGROUND Visceral Leishmaniasis (VL) is an infectious disease that is a significant cause of death among infants aged under 1 year and the elderly in Brazil. Serodiagnosis is a mainstay of VL elimination programs; however, it has significant limitations due to low accuracy. OBJECTIVE This study aime...
Abstract The role of cats in the epidemiological cycle of leishmaniasis remains unclear. To better understand the occurrence of leishmaniasis in cats, we studied the frequency of Leishmania in serum samples of 100 cats living in an endemic region for canine and human leishmaniasis by serological, parasi...
Abstract INTRODUCTION: Herein, we assessed the seroprevalence and spatial distribution of Leishmania infantum in dogs in Garanhuns, Northeastern Brazil. METHODS: Sera samples (n = 242) were analyzed using an enzyme-linked immunosorbent assay (ELISA). The spatial distribution of dogs seropositive for ...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Enfermedades Endémicas/veterinaria,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/inmunología,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Estudios Seroepidemiológicos,
Análisis Espacial
