Leishmaniases comprise a spectrum of diseases caused by protozoan parasites of the genus Leishmania, with some species of rodents being incriminated as reservoirs. The capybara is the largest extant rodent species in the world and is widely distributed in South America. The occurrence of ...
We report the first molecular detection of Leishmania infection (subgenus Viannia) in the yellow-faced parrot (Alipiopsitta xanthops), at a wildlife rehabilitation center located in the city of Campo Grande, Brazil, an endemic area for leishmaniasis. PCRs targeting kinetoplast DNA (kDNA) and the small su...
Abstract Leishmaniasis is a zoonotic disease caused by over 20 species of protozoan parasites of the genus Leishmania. Infection is commonly spread by sandflies and produces a wide spectrum of clinical signs and symptoms. Therefore, from an epidemiological and therapeutic standpoint, it is important to d...
Abstract The present study aimed to evaluate a methodology for active surveillance of visceral leishmaniasis by detecting Leishmania DNA in organs of wild road-killed animals from November 2016 to October 2018 in the North of Paraná, Brazil. The collection points of road-killed wild animals were georefe...
Abstract Canine leishmaniasis (CanL) is a disease caused by Leishmania infantum. Serological methods are the most common diagnostic techniques used for the diagnosis of the CanL. The objective of our study was to estimate the sensitivity and specificity of one in-house ELISA kit (ELISA UNIZAR) and three ...
Anticuerpos Antiprotozoarios/sangre,
Teorema de Bayes,
Enfermedades de los Perros/diagnóstico,
Ensayo de Inmunoadsorción Enzimática/normas,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Análisis de Clases Latentes,
Leishmania infantum/inmunología,
Leishmaniasis/diagnóstico,
Leishmaniasis/veterinaria,
Pruebas Serológicas/normas,
Pruebas Serológicas/veterinaria
Abstract Blood samples and swabs from ocular conjunctiva and mouth were obtained from 64 cats. Of 64 serum samples, 19 were positive for Leishmania antibodies by ELISA (29.80%). Eight cats were positive by PCR (12.5%) in swab samples from mouth and/or ocular mucosa. Poor kappa agreement between serologic...
Anticuerpos Antiprotozoarios/sangre,
Enfermedades de los Gatos/diagnóstico,
Enfermedades de los Gatos/parasitología,
ADN Protozoario/análisis,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania braziliensis/genética,
Leishmania braziliensis/inmunología,
Leishmania braziliensis/aislamiento & purificación,
Leishmania infantum/genética,
Leishmania infantum/inmunología,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis/diagnóstico,
Leishmaniasis/parasitología,
Filogenia,
Reacción en Cadena de la Polimerasa/veterinaria
Bezerra, José Artur Brilhante;
Oliveira, Ilanna Vanessa Pristo de Medeiros;
Yamakawa, Ana Carolina;
Nilsson, Mariana Guimarães;
Tomaz, Klívio Loreno Raulino;
Oliveira, Kalyne Danielly Silva de;
Rocha, Célio Souza da;
Calabuig, Cecília Irene Perez;
Fornazari, Felipe;
Langoni, Helio;
Antunes, João Marcelo Azevedo de Paula.
Abstract The aim of this study was to investigate the occurrence of Leishmania spp. antibodies, and its association with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), in domestic cats from an area endemic for canine and human leishmaniasis in Rio Grande do Norte State, Brazil. Nin...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedades de los Gatos/diagnóstico,
Enfermedades de los Gatos/epidemiología,
Enfermedades de los Gatos/parasitología,
Enfermedades Endémicas,
Técnica del Anticuerpo Fluorescente Directa,
Virus de la Inmunodeficiencia Felina/inmunología,
Leishmaniasis/diagnóstico,
Leishmaniasis/epidemiología,
Leishmaniasis/veterinaria,
Virus de la Leucemia Felina/inmunología,
Reacción en Cadena de la Polimerasa,
Factores de Riesgo
RESUMEN Antecedentes: la fiebre puerperal es una de las complicaciones más comunes que aparece en el puerperio. Objetivo: demostrar cómo el desarrollo de fiebre en el puerperio no siempre está relacionado con el parto. Metodología: se presenta un caso clínico de fiebre puerperal. Resultado: en n...
Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus red...
Anticuerpos Antiprotozoarios/sangre,
Afinidad de Anticuerpos/inmunología,
Biomarcadores/química,
Brasil/epidemiología,
Enfermedad de Chagas/complicaciones,
Enfermedad de Chagas/diagnóstico,
Enfermedad de Chagas/epidemiología,
Enfermedad de Chagas/inmunología,
Ensayo de Inmunoadsorción Enzimática,
Inmunoglobulina G/sangre,
Leishmaniasis/complicaciones,
Leishmaniasis/diagnóstico,
Leishmaniasis/epidemiología,
Leishmaniasis/inmunología,
Vigilancia de la Población,
Sensibilidad y Especificidad,
Urea/química,
Urea/farmacología,
Reacciones Cruzadas/inmunología
