Cloning and heterologous expression of a hydrophobin gene Ltr. hyd from the tiger milk mushroom Lentinus tuber-regium in yeast-like cells of Tremella fuciformis
Electron. j. biotechnol; 32 (), 2018
Background: Hydrophobins are small proteins secreted by filamentous fungi, which show a highly surface activity. Because of the signally self-assembling abilities and surface activities, hydrophobins were considered as candidates in many aspects, for example, stabilizing foams and emulsions in food produ...
Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Clonación Molecular, Lentinula/genética, Lentinula/metabolismo, Basidiomycota/metabolismo, Emulsionantes, Levaduras, Agrobacterium tumefaciens/metabolismo, Southern Blotting, Reacción en Cadena en Tiempo Real de la Polimerasa, Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo, Electroforesis en Gel de Poliacrilamida, Basidiomycota/enzimología, Transformación Genética, Microscopía Fluorescente, Análisis de Secuencia