Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
Braz. j. microbiol; 49 (3), 2018
Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently heal...
Proteínas de la Membrana Bacteriana Externa/genética, Proteínas de la Membrana Bacteriana Externa/orina, Enfermedades de los Perros/diagnóstico, Enfermedades de los Perros/orina, Leptospira/genética, Leptospira/aislamiento & purificación, Leptospirosis/diagnóstico, Leptospirosis/microbiología, Leptospirosis/orina, Leptospirosis/veterinaria, Lipoproteínas/genética, Lipoproteínas/orina, Reacción en Cadena en Tiempo Real de la Polimerasa/métodos, Sensibilidad y Especificidad, Orina/microbiología