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Extracellular expression in Bacillus subtilis of a thermostable Geobacillus stearothermophilus lipase

Elemosho, Ridwan a ,; Suwanto, Antonius a,b,c,⇑; Thenawidjaja, Maggy d.
Electron. j. biotechnol; 53 (), 2021
BACKGROUND The extracellular expression of enzymes in a secretion host such as Bacillus subtilis is a useful strategy in reducing the cost of downstream processing of industrial enzymes. Here, we present the first report of the successful extracellular expression in Bacillus subtilis WB800 of Geobacillus...
Bacillus subtilis/metabolismo, Bacillus subtilis/química, Bacillus subtilis/genética, Bacillus subtilis/aislamiento & purificación, Geobacillus stearothermophilus/metabolismo, Geobacillus stearothermophilus/química, Geobacillus stearothermophilus/genética, Geobacillus stearothermophilus/aislamiento & purificación, Lipasa/química
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Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07

Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Braz. j. microbiol; 47 (3), 2016
ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production...
Acinetobacter/enzimología, Proteínas Bacterianas/biosíntesis, Proteínas Bacterianas/aislamiento & purificación, Lipasa/biosíntesis, Lipasa/aislamiento & purificación, Compuestos Orgánicos, Solventes, Proteínas Bacterianas/química, Cromatografía por Intercambio Iónico, Activación Enzimática, Estabilidad de Enzimas, Espacio Extracelular/enzimología, Concentración de Iones de Hidrógeno, Iones, Cinética, Lipasa/química, Lipólisis, Metales, Peso Molecular, Compuestos Orgánicos/química, Solventes/química, Especificidad por Sustrato, Temperatura
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The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries

Ferreira-Dias, Suzana; Sandoval, Georgina; Plou, Francisco; Valero, Francisco.
Electron. j. biotechnol; 16 (3), 2013
The potential of lipases (E.C.3.1.1.3.) as biocatalysts for the production of fatty acid derivatives for the food and nutraceutical industries, such as flavouring esters, fatty acid esters of antioxidants and structured lipids, is enormous, mainly due to their high regio- and stereo-selectivities, in add...
Lipasa/metabolismo, Lipasa/química, Ácidos Grasos/metabolismo, Suplementos Dietéticos, Industria de Alimentos, Biocatálisis, Alimentos Funcionales, Antioxidantes
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