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Zebrafish ambra1b knockout reveals a novel role for Ambra1 in primordial germ cells survival, sex differentiation and reproduction

Fontana, Camilla Maria; Terrin, Francesca; Facchinello, Nicola; Meneghetti, Giacomo; Dinarello, Alberto; Gambarotto, Lisa; Zuccarotto, Annalisa; Caichiolo, Micol; Brocca, Ginevra; Verin, Ranieri; Nazio, Francesca; Carnevali, Oliana; Cecconi, Francesco; Bonaldo, Paolo; Valle, Luisa Dalla.
Biol. Res; 56 (), 2023
BACKGROUND: AMBRA1 is an intrinsically disordered protein, working as a scaffold molecule to coordinate, by protein-protein interaction, many cellular processes, including autophagy, mitophagy, apoptosis and cell cycle progression. The zebrafish genome contains two ambra1 paralogous genes (a and b), both...
Diferenciación Sexual, Pez Cebra/genética, Pez Cebra/metabolismo, Proteínas Adaptadoras Transductoras de Señales/metabolismo, Células Germinativas/metabolismo, Mamíferos/genética, Mamíferos/metabolismo, ARN Mensajero/metabolismo, Reproducción, Proteínas de Pez Cebra/genética, Proteínas de Pez Cebra/metabolismo
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The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncology

Ehsasatvatan, Maryam; Kohnehrouz, Bahram Baghban; Gholizadeh, Ashraf; Ofoghi, Hamideh; Shanehbandi, Dariush.
Biol. Res; 55 (), 2022
BACKGROUND: Designed mimetic molecules are attractive tools in biopharmaceuticals and synthetic biology. They require mass and functional production for the assessment of upcoming challenges in the near future. The DARPin family is considered a mimetic pharmaceutical peptide group with high affinity bind...
Productos Biológicos, Proteínas de Repetición de Anquirina Diseñadas, Línea Celular Tumoral, Cloroplastos/química, Cloroplastos/metabolismo, Mamíferos/metabolismo, Preparaciones Farmacéuticas/metabolismo, Receptor ErbB-2
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Advances in improving mammalian cells metabolism for recombinant protein production

Altamirano, Claudia; Berrios, Julio; Vergara, Mauricio; Becerra, Silvana.
Electron. j. biotechnol; 16 (3), 2013
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure a...
Proteínas Recombinantes/biosíntesis, Mamíferos/metabolismo, Células/metabolismo, Ingeniería Celular, Células CHO/metabolismo, Glucólisis, Metabolismo Energético, Glutamina/metabolismo
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