Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data
Biol. Res; 49 (), 2016
BACKGROUND: New sequencing technologies have opened the way to the discovery and the characterization of pathogenic viruses in clinical samples. However, the use of these new methods can require an amplification of viral RNA prior to the sequencing. Among all the available methods, the procedure based on...
ARN Polimerasas Dirigidas por ADN/genética, Técnicas de Amplificación de Ácido Nucleico/métodos, Análisis de Secuencia de ARN/métodos, Genoma Viral, ARN Viral, Ensamble de Virus, Alphavirus/genética, Mengovirus/genética, República Centroafricana, Biología Computacional, Mapeo Contig, Valores de Referencia, Reproducibilidad de los Resultados, Programas Informáticos