LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry

Farias, Elizangela; Bezerra, Fhabiane; Baia-da-Silva, Djane Clarys; Chaves, Yury Oliveira; Cardoza, Tatiana Bacry; de Almeida, Maria Edilene Martins; Oliveira, Lucas Barbosa; Lalwani, Pritesh; Orlandi, Patrícia Puccinelli; Lacerda, Marcus Vinicius Guimaraes; Lopes, Stefanie Costa Pinto; Nogueira, Paulo Afonso.

Mem. Inst. Oswaldo Cruz; 114 (), 2019

As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured...

Anticuerpos Antiprotozoarios/inmunología, Citometría de Flujo, Merozoítos/citología, Merozoítos/inmunología, Ratones Endogámicos BALB C, Fagocitosis/fisiología, Plasmodium vivax/inmunología, Plasmodium vivax/fisiología, Proteínas Protozoarias/inmunología

Fulltext

Longitudinal evaluation of humoral immune response and merozoite surface antigen diversity in calves naturally infected with Babesia bovis, in São Paulo, Brazil

Abstract Babesiosis is an economically important infectious disease affecting cattle worldwide. In order to longitudinally evaluate the humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis among naturally infected calves in Taiaçu, Brazil, serum and DNA s...