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Media development and process parameter optimization using statistical experimental designs for the production of nonribosomal peptides in Escherichia coli

Oestreich, Arne Michael; Ilire Suli, Merlinda; Gerlach, Doreen; Fan, Rong; Czermak, Peter.
Electron. j. biotechnol; 52 (), 2021
BACKGROUND: Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and a promising candidate for the synthe...
Escherichia coli, Biotecnología, Péptido Sintasas/metabolismo, Bioingeniería, Modelos Estadísticos, Reactores Biológicos/microbiología, Electroforesis en Gel de Poliacrilamida, Temperatura, Concentración de Iones de Hidrógeno, Carbono/metabolismo
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Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines

Meng, Minjun; Chen, Yanling; Jia, Jianbo; Li, Lianghui; Yang, Sumei.
Biol. Res; 51 (), 2018
BACKGROUND: Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), an enzyme required for de novo purine biosynthesis, is associated with and involved in tumorigenesis. This study aimed to evaluate the role of PAICS in human breast cancer, which rema...
Biomarcadores de Tumor/fisiología, Neoplasias de la Mama/enzimología, Neoplasias de la Mama/patología, Carboxiliasas/biosíntesis, Péptido Sintasas/fisiología, Proliferación Celular, Péptido Sintasas/genética, Línea Celular Tumoral, Citometría de Flujo, Regulación Enzimológica de la Expresión Génica, Regulación Neoplásica de la Expresión Génica, Técnicas de Silenciamiento del Gen
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In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics

Souza, Claudemir; Zanchin, Nilson IT; Krieger, Marco A; Ludwig, Adriana.
Mem. Inst. Oswaldo Cruz; 112 (10), 2017
BACKGROUND The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance o...
Péptido Sintasas, Variación Genética, Genotipo, Pruebas Inmunológicas, Filogenia, Virus Sincitiales Respiratorios, Proteínas Virales/genética
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