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Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil

Albuquerque, Pedro Paulo Feitosa de; Santos, André de Souza; Souza Neto, Orestes Luiz de; Kim, Pomy de Cássia Peixoto; Cavalcanti, Erika Fernanda Torres Samico Fernandes; Oliveira, Júnior Mário Baltazar de; Mota, Rinaldo Aparecido; Pinheiro Júnior, José Wilton.
Braz. j. microbiol; 48 (1), 2017
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collecte...
Microbiología de Alimentos, Leche/microbiología, Mycobacterium avium subsp. paratuberculosis/genética, Brasil, ADN Bacteriano, Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación, Paratuberculosis/diagnóstico, Paratuberculosis/microbiología, Reacción en Cadena de la Polimerasa, Sensibilidad y Especificidad
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Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR

Gilardoni, Liliana Rosa; Fernández, Bárbara; Morsella, Claudia; Mendez, Laura; Jar, Ana María; Paolicchi, Fernando Alberto; Mundo, Silvia Leonor.
Braz. j. microbiol; 47 (2), 2016
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samp...
Enfermedades de los Bovinos/microbiología, Separación Inmunomagnética/métodos, Leche/microbiología, Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación, Paratuberculosis/microbiología, Reacción en Cadena de la Polimerasa/métodos, Argentina, Bovinos, Enfermedades de los Bovinos/diagnóstico, Enfermedades de los Bovinos/fisiopatología, Heces/microbiología, Lactancia, Leche/química, Mycobacterium avium subsp. paratuberculosis/química, Mycobacterium avium subsp. paratuberculosis/genética, Paratuberculosis/diagnóstico, Paratuberculosis/fisiopatología
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