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Heterologous expression and enhanced production of β-1, 4-glucanase of Bacillus halodurans C-125 in Escherichia coli

Zeeshan, Nadia; Naz, Saher; Naz, Shumaila; Afroz, Amber; Zahur, Muzna; Zia, Safia.
Electron. j. biotechnol; 34 (), 2018
Background: Recombinant DNA technology enables us to produce proteins with desired properties and insubstantial amount for industrial applications. Endo-1, 4-β-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study w...
Bacillus/enzimología, Escherichia coli/metabolismo, Celulasas/biosíntesis, Expresión Génica, Hidrólisis, Reacción en Cadena de la Polimerasa, Clonación Molecular, Celulasas/metabolismo, Celulasas/aislamiento & purificación, Células Vegetales/enzimología, Pared Celular/enzimología, Estabilidad de Enzimas, Concentración de Iones de Hidrógeno, Temperatura
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Crecimiento in vitro y producción de enzimas degradadoras de pared celular vegetal de aislamientos argentinos de Macrophomina phaseolina, agente causal de la podredumbre carbonosa en maíz
In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn

Ramos, Araceli M; Gally, Marcela; Szapiro, Gala; Itzcovich, Tatiana; Carabajal, Maira; Levin, Laura.
Rev. argent. microbiol; 48 (4), 2016
Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non...
Zea mays/parasitología, Zea mays/enzimología, Poligalacturonasa/aislamiento & purificación, Celulasa/aislamiento & purificación, Endo-1,4-beta Xilanasas/aislamiento & purificación, Técnicas In Vitro/métodos, Pared Celular/enzimología
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