Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast
Electron. j. biotechnol; 18 (3), 2015
Background The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidas...
Poligalacturonasa/metabolismo, Poligalacturonasa/genética, Peroxidasa/metabolismo, Peroxidasa/genética, Cloroplastos/genética, Nicotiana, Hongos/enzimología, Bacterias/enzimología, Pared Celular, Transformación Genética, Hidrolasas, Reacción en Cadena de la Polimerasa, Temperatura, Concentración de Iones de Hidrógeno, Southern Blotting