Background: Methanol can be effectively removed from air by biofiltration. However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms, and it can be released out of the cell constituting a secondary emission. Results: The total remova...
ABSTRACT The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and...
Medios de Cultivo/química,
Medios de Cultivo/metabolismo,
Ácidos Grasos no Esterificados/metabolismo,
Fermentación,
Glicerol/metabolismo,
Metanol/metabolismo,
Pichia/genética,
Pichia/crecimiento & desarrollo,
Pichia/metabolismo,
Proteínas Recombinantes/genética,
Temperatura
Background: α-Amylase is widely used in the starch processing, food and paper industries, hydrolyzing starch, glycogen and other polysaccharides into glucose, maltose and oligosaccharides. An α-amylase gene family from Aspergillus niger CBS513.88 encode eight putative α-amylases. The differences and s...
Aspergillus niger/enzimología,
alfa-Amilasas/genética,
alfa-Amilasas/química,
Hidrólisis,
Pichia/metabolismo,
Industria de Alimentos,
Almidón,
Clonación Molecular,
Fermentación,
Temperatura,
Concentración de Iones de Hidrógeno
Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole...
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically m...
Anticuerpos/genética,
Anticuerpos/metabolismo,
Carbono/metabolismo,
Medios de Cultivo/química,
Medios de Cultivo/metabolismo,
Fermentación,
Glicerol/metabolismo,
Microbiología Industrial/métodos,
Lipoproteínas LDL/inmunología,
Pichia/genética,
Pichia/crecimiento & desarrollo,
Pichia/metabolismo,
Proteínas Recombinantes/genética,
Proteínas Recombinantes/metabolismo,
Anticuerpos de Cadena Única/biosíntesis,
Anticuerpos de Cadena Única/genética
Abstract The application of high-potential thermotolerant yeasts is a key factor for successful ethanol production at high temperatures. Two hundred and thirty-four yeast isolates from Greater Mekong Subregion (GMS) countries, i.e., Thailand, The Lao People's Democratic Republic (Lao PDR) and Vietnam wer...
Asia Sudoriental,
Etanol/metabolismo,
Glucosa/metabolismo,
Calor,
Pichia/química,
Pichia/genética,
Pichia/aislamiento & purificación,
Pichia/metabolismo,
Saccharomyces cerevisiae/química,
Saccharomyces cerevisiae/genética,
Saccharomyces cerevisiae/aislamiento & purificación,
Saccharomyces cerevisiae/metabolismo,
Sorghum/metabolismo
Background: Laccases are copper-containing enzymes which have been used as green biocatalysts for many industrial processes. Although bacterial laccases have high stabilities which facilitate their application under harsh conditions, their activities and production yields are usually very low. In this wo...
Pichia/metabolismo,
Lacasa/biosíntesis,
Lacasa/genética,
Mutagénesis,
Lacasa/metabolismo,
Bacillus licheniformis/enzimología,
Catálisis,
Colorantes/metabolismo,
Estabilidad de Enzimas,
Levaduras,
Temperatura,
Concentración de Iones de Hidrógeno
Abstract This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into t...
Factor de Crecimiento Epidérmico/metabolismo,
Pichia/metabolismo,
Proteínas Recombinantes/metabolismo,
Clonación Molecular,
Medios de Cultivo/química,
Ensayo de Inmunoadsorción Enzimática,
Factor de Crecimiento Epidérmico/genética,
Fermentación,
Expresión Génica,
Concentración de Iones de Hidrógeno,
Pichia/genética,
Proteínas Recombinantes/genética
Background: Invert sugar is used greatly in food and pharmaceutical industries. This paper describes scaling-up batch conditions for sucrose inversion catalyzed by the recombinant Pichia pastoris BfrA4X whole cells expressing Thermotoga maritima invertase entrapped in calcium alginate beads. For the firs...
Pichia/metabolismo,
Pichia/citología,
Hidrólisis,
Biotecnología/métodos,
Sacarosa/metabolismo,
Sacarosa/química,
Reactores Biológicos,
Biocatálisis,
Enzimas Inmovilizadas,
Cinética,
Alginatos,
Thermotoga maritima/enzimología
Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing ne...
Antígenos de Protozoos/biosíntesis,
Antígenos de Protozoos/sangre,
Antígenos de Protozoos/inmunología,
Antígenos de Superficie/biosíntesis,
Antígenos de Superficie/sangre,
Antígenos de Superficie/inmunología,
Enfermedades de los Perros/sangre,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/parasitología,
Ensayo de Inmunoadsorción Enzimática,
Neospora/inmunología,
Pichia/metabolismo,
Infecciones Protozoarias en Animales/sangre,
Infecciones Protozoarias en Animales/diagnóstico,
Proteínas Protozoarias/biosíntesis,
Proteínas Protozoarias/sangre,
Proteínas Protozoarias/inmunología,
Ovinos,
Enfermedades de las Ovejas/sangre,
Enfermedades de las Ovejas/diagnóstico,
Enfermedades de las Ovejas/parasitología
