LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Development of biodegradation process for Poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1

Panyachanakul, Titiporn; Sorachart, Bodeesorn; Lumyong, Saisamorn; Lorliam, Wanlapa; Kitpreechavanich, Vichien; Krajangsang, Sukhumaporn.

Electron. j. biotechnol; 40 (), 2019

Background: Plastic waste is a serious problem because it is difficult to degrade, thereby leading to global environment problems. Poly(lactic acid) (PLA) is a biodegradable aliphatic polyester derived from renewable resources, and it can be degraded by various enzymes produced by microorganisms. This st...

Actinomycetales/enzimología, Polímeros/metabolismo, Poliésteres/metabolismo, Biodegradación Ambiental, Ácido Láctico/análisis, Reactores Biológicos, Enzimas/metabolismo, Concentración de Iones de Hidrógeno

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Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes

Panyachanakul, Titiporn; Kitpreechavanich, Vichien; Tokuyama, Shinji; Krajangsang, Sukhumaporn.

Electron. j. biotechnol; 30 (), 2017

Background: Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading e...

Actinomycetales/enzimología, Enzimas Inmovilizadas, Fermentación, Poliésteres/metabolismo, Enzimas/biosíntesis, Enzimas/metabolismo, Reactores Biológicos, Biodegradación Ambiental

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A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry

Alves, LPS; Almeida, AT; Cruz, LM; Pedrosa, FO; de Souza, EM; Chubatsu, LS; Müller-Santos, M; Valdameri, G.

Braz. j. med. biol. res; 50 (1), 2017

The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) pro...

Azospirillum brasilense/metabolismo, Citometría de Flujo/métodos, Herbaspirillum/metabolismo, Hidroxibutiratos/metabolismo, Microscopía Fluorescente, Raíces de Plantas/microbiología, Poliésteres/metabolismo

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