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Production and evaluation of egg derived hot start antibodies

Spyrydonov, Vladyslav; Pihida, Dmitro; Sereda, Alexander; Likhanov, Artur; Yu, Weiming.
Electron. j. biotechnol; 44 (), 2020
BACKGROUND: Hot start can greatly improve specificity, sensitivity and yield of PCR. Non-specific amplification can occur in PCR when reaction mixture is prepared at room temperature, because Taq DNA polymerase is active and the primers can hybridize non-specifically. Hot start Taq DNA polymerases remain...
Anticuerpos Monoclonales/inmunología, Yema de Huevo/inmunología, Temperatura, Ensayo de Inmunoadsorción Enzimática, Polimerasa Taq, Inmunoglobulinas/inmunología, Reacción en Cadena de la Polimerasa, Anticuerpos Monoclonales/aislamiento & purificación, Inmunoglobulinas/aislamiento & purificación, Yema de Huevo/metabolismo, Anticuerpos Monoclonales/biosíntesis, Inmunoglobulinas/biosíntesis
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A simple and efficient method for extraction of Taq DNA polymerase

Chen, Sique; Zheng, Xiujuan; Cao, Hongrui; Jiang, Linghui; Liu, Fangqian; Sun, Xinli.
Electron. j. biotechnol; 18 (5), 2015
Background Thermostable DNA polymerase (Taq Pol ?) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. Moreover, we found that 30-40% activity of Taq...
Polimerasa Taq/genética, Polimerasa Taq/aislamiento & purificación, Reacción en Cadena de la Polimerasa, Etanol/química, Precipitación Química
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Monitoramento de doença residual mínima em leucemia mielóide crônica por PCR em tempo real: [revisão]
Monitoring minimal residual disease in chronic myeloid leukemia by real time PCR: [review]

Almeida, Priscilla S. R; Saddi, Vera Aparecida.
Rev. bras. hematol. hemoter; 29 (4), 2007
O monitoramento de doença residual mínima (DRM) em leucemia mielóide crônica é extremamente importante, pois possibilita o diagnóstico precoce de eventuais recidivas da doença. Este estudo visa apresentar uma revisão bibliográfica sobre a aplicação e a avaliação da eficácia do método de PC...
Diagnóstico Precoz, Leucemia Mielógena Crónica BCR-ABL Positiva, Neoplasia Residual, Reacción en Cadena en Tiempo Real de la Polimerasa, Recurrencia, Polimerasa Taq, Transcripción Reversa, Trasplante de Células Madre, Trasplante de Médula Ósea
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Production of recombinant enzymes of wide use for research

Manzur, María J; Muñoz, Rosana V; Lucero, Adrián A; Juri Ayub, Maximiliano; Alvarez, Sergio E; Ciuffo, Gladys M.
Electron. j. biotechnol; 9 (3), 2006
For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present paper,...
Virus de la Leucemia Murina de Moloney/enzimología, Polimerasa Taq/metabolismo, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa, Escherichia coli/enzimología, Proteínas Recombinantes/biosíntesis, Biotecnología/métodos, ADN Polimerasa Dirigida por ARN/biosíntesis, Temperatura, ADN Polimerasa Dirigida por ADN/aislamiento & purificación, Proteínas Recombinantes/aislamiento & purificación
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