Results: 7

Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process

Vieira, Gabriela A. L; Magrini, Mariana Juventina; Bonugli-Santos, Rafaella C; Rodrigues, Marili V. N; Sette, Lara D.

Braz. j. microbiol; 49 (4), 2018

ABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental po...

Basidiomycota/clasificación, Basidiomycota/genética, Basidiomycota/aislamiento & purificación, Basidiomycota/metabolismo, Benzo(a)pireno/química, Benzo(a)pireno/metabolismo, Biodegradación Ambiental, Sistema Enzimático del Citocromo P-450/genética, Sistema Enzimático del Citocromo P-450/metabolismo, Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Filogenia, Hidrocarburos Policíclicos Aromáticos/química, Hidrocarburos Policíclicos Aromáticos/metabolismo, Pirenos/química, Pirenos/metabolismo, Agua de Mar/microbiología

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Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach

Souza, Renata Carolini; Cantão, Maurício Egídio; Nogueira, Marco Antonio; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela.

Braz. j. microbiol; 49 (4), 2018

ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional ...

Agricultura, Archaea/clasificación, Archaea/enzimología, Archaea/genética, Archaea/aislamiento & purificación, Proteínas Arqueales/genética, Brasil, Proteínas Fúngicas/genética, Hongos/clasificación, Hongos/enzimología, Hongos/genética, Hongos/aislamiento & purificación, Hidrolasas/genética, Metagenoma, Metagenómica, Suelo/química, Microbiología del Suelo, Glycine max/crecimiento & desarrollo, Triticum/crecimiento & desarrollo, Zea mays/crecimiento & desarrollo

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Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis

Rungrattanakasin, Budsayachat; Premjet, Siripong; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.

Braz. j. microbiol; 49 (3), 2018

Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and enc...

Aspergillus fumigatus/enzimología, Aspergillus fumigatus/genética, Celulasa/química, Celulasa/genética, Celulasa/metabolismo, Clonación Molecular, Estabilidad de Enzimas, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Expresión Génica, Calor, Concentración de Iones de Hidrógeno, Kluyveromyces/genética, Kluyveromyces/metabolismo, Proteínas Recombinantes/química, Proteínas Recombinantes/genética, Proteínas Recombinantes/metabolismo, Especificidad por Sustrato

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Cloning and heterologous expression of a hydrophobin gene Ltr. hyd from the tiger milk mushroom Lentinus tuber-regium in yeast-like cells of Tremella fuciformis

Liu, Dongmei; Zhu, Hanyu; Chen, Yue; Zheng, Liesheng; Chen, Liguo; Ma, Aimin.

Electron. j. biotechnol; 32 (), 2018

Background: Hydrophobins are small proteins secreted by filamentous fungi, which show a highly surface activity. Because of the signally self-assembling abilities and surface activities, hydrophobins were considered as candidates in many aspects, for example, stabilizing foams and emulsions in food produ...

Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Clonación Molecular, Lentinula/genética, Lentinula/metabolismo, Basidiomycota/metabolismo, Emulsionantes, Levaduras, Agrobacterium tumefaciens/metabolismo, Southern Blotting, Reacción en Cadena en Tiempo Real de la Polimerasa, Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo, Electroforesis en Gel de Poliacrilamida, Basidiomycota/enzimología, Transformación Genética, Microscopía Fluorescente, Análisis de Secuencia

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Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici

Hernández-Monjaraz, Wendy Susana; Caudillo-Pérez, César; Salazar-Sánchez, Pedro Ulises; Macías-Sánchez, Karla Lizbeth.

Braz. j. microbiol; 49 (supl.1), 2018

ABSTRACT Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic a...

Cobre/metabolismo, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Fusarium/química, Fusarium/enzimología, Fusarium/genética, Hierro/metabolismo, Lacasa/química, Lacasa/genética, Lacasa/metabolismo, Solanum lycopersicum/microbiología, Enfermedades de las Plantas/microbiología

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Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability

Fonseca, María Isabel; Tejerina, Marcos Raúl; Sawostjanik-Afanasiuk, Silvana Soledad; Giorgio, Ernesto Martin; Barchuk, Mónica Lucrecia; Zapata, Pedro Darío; Villalba, Laura Lidia.

Braz. j. microbiol; 47 (2), 2016

Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purpos...

Proteínas Fúngicas/metabolismo, Lacasa/metabolismo, Trametes/enzimología, Argentina, Estabilidad de Enzimas, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Lacasa/química, Lacasa/genética, Temperatura, Trametes/genética, Trametes/aislamiento & purificación

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Isolation and expression of two polyketide synthase genes from Trichoderma harzianum 88 during mycoparasitism

Yao, Lin; Tan, Chong; Song, Jinzhu; Yang, Qian; Yu, Lijie; Li, Xinling.

Braz. j. microbiol; 47 (2), 2016

Abstract Metabolites of mycoparasitic fungal species such as Trichoderma harzianum 88 have important biological roles. In this study, two new ketoacyl synthase (KS) fragments were isolated from cultured Trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. The ...

Proteínas Fúngicas/metabolismo, Sintasas Poliquetidas/metabolismo, Trichoderma/enzimología, Secuencia de Aminoácidos, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Regulación Fúngica de la Expresión Génica, Datos de Secuencia Molecular, Micelio/enzimología, Micelio/genética, Enfermedades de las Plantas/microbiología, Sintasas Poliquetidas/química, Sintasas Poliquetidas/genética, Alineación de Secuencia, Trichoderma/clasificación, Trichoderma/genética

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