Results: 6

Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

Cardoso, Wilton Soares; Queiroz, Paula Viana; Tavares, Gabriella Peterlini; Santos, Fernando Almeida; Soares, Filippe Elias de Freitas; Kasuya, Maria Catarina Megumi; Queiroz, José Humberto de.

Braz. j. microbiol; 49 (4), 2018

ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commer...

Biocatálisis, Celulasas/química, Celulasas/metabolismo, Proteínas Fúngicas/química, Proteínas Fúngicas/metabolismo, Hongos/química, Hongos/enzimología, Hidrólisis, Lignina/química, Lignina/metabolismo, Tallos de la Planta/química, Tallos de la Planta/microbiología, Sorghum/química, Sorghum/microbiología

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Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis

Rungrattanakasin, Budsayachat; Premjet, Siripong; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.

Braz. j. microbiol; 49 (3), 2018

Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and enc...

Aspergillus fumigatus/enzimología, Aspergillus fumigatus/genética, Celulasa/química, Celulasa/genética, Celulasa/metabolismo, Clonación Molecular, Estabilidad de Enzimas, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Expresión Génica, Calor, Concentración de Iones de Hidrógeno, Kluyveromyces/genética, Kluyveromyces/metabolismo, Proteínas Recombinantes/química, Proteínas Recombinantes/genética, Proteínas Recombinantes/metabolismo, Especificidad por Sustrato

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Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

Wang, Xiuwen; Zhang, Xiaohua; Yao, Qingshou; Hua, Dongliang; Qin, Jiayang.

Braz. j. microbiol; 49 (supl.1), 2018

Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of scl...

Ascomicetos/química, Ascomicetos/genética, Ascomicetos/metabolismo, Carbono/metabolismo, Diterpenos/metabolismo, Electroforesis en Gel Bidimensional, Proteínas Fúngicas/química, Proteínas Fúngicas/metabolismo, Regulación Fúngica de la Expresión Génica, Glucosa/metabolismo, Proteómica

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Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici

Hernández-Monjaraz, Wendy Susana; Caudillo-Pérez, César; Salazar-Sánchez, Pedro Ulises; Macías-Sánchez, Karla Lizbeth.

Braz. j. microbiol; 49 (supl.1), 2018

ABSTRACT Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic a...

Cobre/metabolismo, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Proteínas Fúngicas/metabolismo, Fusarium/química, Fusarium/enzimología, Fusarium/genética, Hierro/metabolismo, Lacasa/química, Lacasa/genética, Lacasa/metabolismo, Solanum lycopersicum/microbiología, Enfermedades de las Plantas/microbiología

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Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability

Fonseca, María Isabel; Tejerina, Marcos Raúl; Sawostjanik-Afanasiuk, Silvana Soledad; Giorgio, Ernesto Martin; Barchuk, Mónica Lucrecia; Zapata, Pedro Darío; Villalba, Laura Lidia.

Braz. j. microbiol; 47 (2), 2016

Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purpos...

Proteínas Fúngicas/metabolismo, Lacasa/metabolismo, Trametes/enzimología, Argentina, Estabilidad de Enzimas, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Lacasa/química, Lacasa/genética, Temperatura, Trametes/genética, Trametes/aislamiento & purificación

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Isolation and expression of two polyketide synthase genes from Trichoderma harzianum 88 during mycoparasitism

Yao, Lin; Tan, Chong; Song, Jinzhu; Yang, Qian; Yu, Lijie; Li, Xinling.

Braz. j. microbiol; 47 (2), 2016

Abstract Metabolites of mycoparasitic fungal species such as Trichoderma harzianum 88 have important biological roles. In this study, two new ketoacyl synthase (KS) fragments were isolated from cultured Trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. The ...

Proteínas Fúngicas/metabolismo, Sintasas Poliquetidas/metabolismo, Trichoderma/enzimología, Secuencia de Aminoácidos, Proteínas Fúngicas/química, Proteínas Fúngicas/genética, Regulación Fúngica de la Expresión Génica, Datos de Secuencia Molecular, Micelio/enzimología, Micelio/genética, Enfermedades de las Plantas/microbiología, Sintasas Poliquetidas/química, Sintasas Poliquetidas/genética, Alineación de Secuencia, Trichoderma/clasificación, Trichoderma/genética

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