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Transient expression of a green fluorescent protein in tobacco and maize chloroplast

Arévalo Gallegos, Sigifredo; Varela Rodríguez, Hugo; Lugo Aguilar, Héctor; Siqueiros Cendón, Tania S; Iglesias Figueroa, Blanca F; Espinoza Sánchez, Edward A; Aguado Santacruz, Gerardo A; Rascón Cruz, Quintín.
Electron. j. biotechnol; 45 (), 2020
BACKGROUND: Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant c...
Zea mays/genética, Cloroplastos/genética, Cloroplastos/metabolismo, Proteínas Fluorescentes Verdes/metabolismo, Nicotiana/metabolismo, Proteínas Fluorescentes Verdes/genética, Plastidios/genética, Plantas Modificadas Genéticamente, Biotecnología, Transformación Genética, Reacción en Cadena de la Polimerasa, Escherichia coli, Genoma del Cloroplasto
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Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

Cortesia, Claudia; Bello, Teresita; Lopez, Gustavo; Franzblau, Scott; Waard, Jacobus de; Takiff, Howard.
Braz. j. microbiol; 48 (1), 2017
Abstract Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recentl...
Antibacterianos/farmacología, Desinfectantes/farmacología, Expresión Génica, Proteínas Fluorescentes Verdes, Micobacterias no Tuberculosas/efectos de los fármacos, Compuestos de Amonio Cuaternario/farmacología, Relación Dosis-Respuesta a Droga, Proteínas Fluorescentes Verdes/genética, Pruebas de Sensibilidad Microbiana, Micobacterias no Tuberculosas/clasificación, Micobacterias no Tuberculosas/genética, Plásmidos/genética, Proteínas Recombinantes de Fusión/genética
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