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Fixation of pfcrt chloroquine resistance alleles in Plasmodium falciparum clinical isolates collected from unrest tribal agencies of Pakistan

Nadeem, M F; Zeeshan, N; Khattak, A A; Awan, U A; Yaqoob, A.
Braz. j. biol; 83 (), 2023
Abstract Plasmodium falciparum resistance to Chloroquine (CQ) is a significant cause of mortality and morbidity worldwide. There is a paucity of documented data on the prevalence of CQ-resistant mutant haplotypes of Pfcrt and Pfmdr1 genes from malaria-endemic war effected Federally Administered Tribal Ar...
Alelos, Antimaláricos/farmacología, Cloroquina/farmacología, Resistencia a Medicamentos/genética, Proteínas de Transporte de Membrana/genética, Proteínas Asociadas a Resistencia a Múltiples Medicamentos/genética, Pakistán, Plasmodium falciparum/genética, Proteínas Protozoarias/genética
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PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan

Ullah, I; Afridi, S G; Khan, A U; Israr, M; Ali, A; Shams, S; Jabeen, H; Rasool, A; Akbar, F; Rahat, M A; Haris, M; Khan, A; Siraj, M; Shah, M.
Braz. j. biol; 82 (), 2022
Plasmodium vivax is the most common human malaria parasite in Asian countries including Pakistan. Present study was designed to explore the genetic diversity of plasmodium vivax genotypes based on Pvmsp-3α and Pvmsp-3βgenes using allelic specific nested PCR and RFLP assays markers from field isolates i...
Variación Genética, Genotipo, Pakistán, Plasmodium vivax/genética, Reacción en Cadena de la Polimerasa, Polimorfismo de Longitud del Fragmento de Restricción, Proteínas Protozoarias/genética
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Plasmodium vivax metacaspase 1 (PvMCA1) catalytic domain is conserved in field isolates from Brazilian Amazon

de Souza, Hugo Amorim dos Santos; Escafa, Victor Fernandes; Blanco, Carolina Moreira; Baptista, Bárbara de Oliveira; de Barros, Jenifer Peixoto; Riccio, Evelyn Ketty Pratt; Rodrigues, Aline Beatriz Mello; Melo, Gisely Cardoso de; Lacerda, Marcus Vinícius Guimarães de; de Souza, Rodrigo Medeiros; Lima-Junior, Josué da Costa; Guimarães, Ana Carolina Ramos; da Mota, Fabio Faria; da Silva, João Hermínio Martins; Daniel-Ribeiro, Cláudio Tadeu; Pratt-Riccio, Lilian Rose; Totino, Paulo Renato Rivas.
Mem. Inst. Oswaldo Cruz; 116 (), 2021
In the present study, we investigated the genetic diversity of Plasmodium vivax metacaspase 1 (PvMCA1) catalytic domain in two municipalities of the main malaria hotspot in Brazil, i.e., the Juruá Valley, and observed complete sequence identity among all P. vivax field isolates and the Sal-1 reference s...
Brasil, Dominio Catalítico, Variación Genética/genética, Malaria Vivax, Plasmodium vivax/genética, Proteínas Protozoarias/genética
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Mucosal leishmaniasis: A forgotten disease, description and identification of species in 50 Colombian cases

Muvdi-Arenas, Sandra; Ovalle-Bracho, Clemencia.
Biomédica (Bogotá); 39 (supl.2), 2019
Abstract Introduction: Mucosal leishmaniasis has a progressive course and can cause deformity and even mutilation in the affected areas. It is endemic in the American continent and it is mainly caused by Leishmania (Viannia) braziliensis. Objective: To describe a series of mucosal leishmaniasis cases a...
Leishmania braziliensis/aislamiento & purificación, Leishmania guyanensis/aislamiento & purificación, Leishmaniasis Mucocutánea/parasitología, Colombia/epidemiología, ADN Protozoario/genética, Genes Protozoarios, Proteínas HSP70 de Choque Térmico/genética, Leishmania braziliensis/clasificación, Leishmania braziliensis/genética, Leishmania guyanensis/clasificación, Leishmania guyanensis/genética, Leishmaniasis Mucocutánea/complicaciones, Leishmaniasis Mucocutánea/epidemiología, Leishmaniasis Mucocutánea/patología, Reacción en Cadena de la Polimerasa, Polimorfismo de Longitud del Fragmento de Restricción, Proteínas Protozoarias/genética, Análisis de Secuencia de ADN, Piel/parasitología, Especificidad de la Especie
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Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Chapadense, Francesca; Machado, Ricardo Luiz Dantas; Ventura, Ana Maria Revorêdo da Silva; Áreas, André; Machado, Renato Beilner; Viana, Giselle Rachid; Zalis, Mariano Gustavo; Castro, Ana Maria de; Cravo, Pedro.
Rev. Soc. Bras. Med. Trop; 52 (), 2019
Abstract INTRODUCTION Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazi...
Artemisininas/farmacología, Resistencia a Medicamentos/genética, Genotipo, Malaria Falciparum/parasitología, Mutación/genética, Fenotipo, Plasmodium falciparum/efectos de los fármacos, Plasmodium falciparum/genética, Proteínas Protozoarias/genética
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Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes

Batista, Cassiano Martin; Saad, Felipe; Ceccoti, Stephane Pini Costa; Eger, Iriane; Soares, Maurilio José.
Mem. Inst. Oswaldo Cruz; 113 (8), 2018
Dynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs). A putative PAT (TcPAT1) has been previously identified in Trypanosoma cruzi, the etiological agent of Chagas disease. Here we analys...
Regulación de la Expresión Génica, Lipoilación/genética, Palmitatos/metabolismo, Proteína S/genética, Proteína S/metabolismo, Proteínas Protozoarias/genética, Proteínas Protozoarias/metabolismo, Trypanosoma cruzi/enzimología, Trypanosoma cruzi/genética, Trypanosoma cruzi/metabolismo
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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo, Gabriel Alves; Patané, José Salvatore Leister; Silva, Dalton Giovanni Nogueira da; Junqueira-de-Azevedo, Inácio Loiola Meirelles; Mendonca, Ronaldo Zucatelli; Simons, Simone Michaela; Carvalho, Eneas de; Barros-Battesti, Darci Moraes.
Rev. bras. parasitol. vet; 26 (2), 2017
Abstract Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also harmful to humans. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing inf...
Perfilación de la Expresión Génica/métodos, Perfilación de la Expresión Génica/veterinaria, Mucosa Intestinal/metabolismo, Ornithodoros/clasificación, Ornithodoros/metabolismo, Filogenia, Proteínas Protozoarias/genética, Proteínas Protozoarias/metabolismo
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Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis

Aguiar, Juliana Martins; Silva, Sheila Oliveira; Santos, Valdir Azevedo dos; Taniwaki, Sueli Akemi; Oliveira, Tricia Maria Ferreira de Sousa; Ferreira, Helena Lage; Keid, Lara Borges; Gregori, Fábio; Soares, Rodrigo Martins.
Rev. bras. parasitol. vet; 25 (2), 2016
Abstract Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitud...
Alelos, Quistes/genética, Proteínas del Citoesqueleto/genética, Tamización de Portadores Genéticos/veterinaria, Genotipo, Giardia lamblia/clasificación, Giardia lamblia/genética, Proteínas Protozoarias/genética
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