LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis

Nogueira, Camila Tita; Cistia, Mayara Lúcia Del; Urbaczek, Ana Carolina; Jusi, Márcia MG; Velásquez, Angela Maria Arenas; Machado, Rosângela Zacarias; Ferreira, Henrique; Henrique-Silva, Flávio; Langoni, Hélio; Costa, Paulo Inácio da; Graminha, Márcia AS.

Mem. Inst. Oswaldo Cruz; 113 (3), 2018

Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensiti...

Enfermedades de los Perros/diagnóstico, Electroforesis en Gel de Poliacrilamida/veterinaria, Ensayo de Inmunoadsorción Enzimática/veterinaria, Leishmania infantum/inmunología, Leishmaniasis Visceral/diagnóstico, Leishmaniasis Visceral/veterinaria, Proteínas Protozoarias/sangre, Sensibilidad y Especificidad

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Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological test

Jusi, Márcia Mariza Gomes; Oliveira, Trícia Maria Ferreira de Sousa; Nakaghi, Andréa Cristina Higa; André, Marcos Rogério; Machado, Rosangela Zacarias.

Rev. bras. parasitol. vet; 24 (3), 2015

This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this protein as an antigen in serological assays; and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three di...

Antígenos de Protozoos/sangre, Enfermedades de los Perros/sangre, Enfermedades de los Perros/diagnóstico, Leishmania infantum/inmunología, Leishmania infantum/metabolismo, Leishmaniasis Visceral/sangre, Leishmaniasis Visceral/veterinaria, Proteínas Protozoarias/biosíntesis, Proteínas Protozoarias/sangre, Proteínas Recombinantes/biosíntesis, Proteínas Recombinantes/análisis, Pruebas Serológicas

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Use of ELISA based on NcSRS2 of Neospora caninum expressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs

Pinheiro, Amanda Fernandes; Borsuk, Sibele; Berne, Maria Elisabeth Aires; Pinto, Luciano da Silva; Andreotti, Renato; Roos, Talita; Roloff, Barbara Couto; Leite, Fábio Pereira Leivas.

Rev. bras. parasitol. vet; 24 (2), 2015

Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing ne...

Antígenos de Protozoos/biosíntesis, Antígenos de Protozoos/sangre, Antígenos de Protozoos/inmunología, Antígenos de Superficie/biosíntesis, Antígenos de Superficie/sangre, Antígenos de Superficie/inmunología, Enfermedades de los Perros/sangre, Enfermedades de los Perros/diagnóstico, Enfermedades de los Perros/parasitología, Ensayo de Inmunoadsorción Enzimática, Neospora/inmunología, Pichia/metabolismo, Infecciones Protozoarias en Animales/sangre, Infecciones Protozoarias en Animales/diagnóstico, Proteínas Protozoarias/biosíntesis, Proteínas Protozoarias/sangre, Proteínas Protozoarias/inmunología, Ovinos, Enfermedades de las Ovejas/sangre, Enfermedades de las Ovejas/diagnóstico, Enfermedades de las Ovejas/parasitología

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