L-Asparaginase catalysing the breakdown of L-Asparagine to L-Aspartate and ammonia is an enzyme of therapeutic importance in the treatment of cancer, especially the lymphomas and leukaemia. The present study describes the recombinant production, properties and anticancer potential of enzyme from a hypert...
Antineoplásicos/farmacología,
Asparaginasa/biosíntesis,
Asparaginasa/farmacología,
Células CACO-2,
Estabilidad de Enzimas,
Escherichia coli/genética,
Concentración de Iones de Hidrógeno,
Simulación del Acoplamiento Molecular,
Pyrococcus abyssi/enzimología,
Proteínas Recombinantes/biosíntesis,
Proteínas Recombinantes/farmacología,
Especificidad por Sustrato
BACKGROUND: Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as apoptosis engineering. Previously, it is reported that t...
Chloroplast biotechnology has emerged as a promissory platform for the development of modified plants to express products aimed mainly at the pharmaceutical, agricultural, and energy industries. This technology’s high value is due to its high capacity for the mass production of proteins. Moreover, the ...
The enrichment of therapeutic protein production yield in mammalian cell cultures by modulating mRNA stability is a fairly new strategy in biotechnological applications. Here, we describe the application of 3′-untranslated region (3′UTR) from RNA viral genome to modulate mRNA stability.The data obtai...
Background: P64k is a Neisseria meningitidis high molecular weight protein present in meningococcal vaccine preparations. The lpdA gene, which encodes for this protein, was cloned in Escherichia coli and the P64k recombinant protein was expressed in E. coli K12 GC366 cells under the control of a tryptoph...
Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole...
Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recom...
This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this protein as an antigen in serological assays; and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three di...
Antígenos de Protozoos/sangre,
Enfermedades de los Perros/sangre,
Enfermedades de los Perros/diagnóstico,
Leishmania infantum/inmunología,
Leishmania infantum/metabolismo,
Leishmaniasis Visceral/sangre,
Leishmaniasis Visceral/veterinaria,
Proteínas Protozoarias/biosíntesis,
Proteínas Protozoarias/sangre,
Proteínas Recombinantes/biosíntesis,
Proteínas Recombinantes/análisis,
Pruebas Serológicas
Background: Recombinant proteins, including antibodies and antibody fragments, often contain disulfide bond bridges that are necessary for their folding, stability and function. Production of disulfide-bond-containing proteins in the periplasm of Escherichia coli has been very useful, due to unique chara...
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure a...
