BACKGROUND: Spermatogonial stem cells (SSCs) are critical for sustaining spermatogenesis. Even though several regulators of SSC have been identified in rodents, the regulatory mechanism of SSC in humans has yet to be discovered. METHODS: To explore the regulatory mechanisms of human SSCs, we analyzed pub...
Background: It is important to detect novel biomarkers responsible for the progression and spread of colorectal cancer (CRC) to better evaluate the prognosis of the patients, provide better management, and foster the development of therapeutic targets. In humans, pyrroline-5-carboxylate reductase 2 (PYCR...
BACKGROUND: Aerobic metabolism generates reactive oxygen species that may cause critical harm to the cell. The aim of this study is the characterization of the stress responses in the model aromatic degrading bacterium Paraburkholderia xenovorans LB400 to the oxidizing agents paraquat and H 2 O2. METHODS...
Proteínas de Escherichia coli/genética,
Proteínas de Escherichia coli/metabolismo,
Proteínas Bacterianas/genética,
Proteínas Bacterianas/metabolismo,
Escherichia coli/genética,
Regulación Bacteriana de la Expresión Génica,
Peróxido de Hidrógeno/farmacología,
Proteínas Represoras/genética,
Proteínas Represoras/metabolismo,
Espectrometría de Masas en Tándem,
Estrés Oxidativo,
Oxidación-Reducción,
Cromatografía Liquida,
Burkholderiaceae
ABSTRACT Background: Decreased levels of repressor element-1 silencing transcription (REST) factor in the brain, plasma, and neuron-derived exosomes are associated with Alzheimers disease (AD). Objective: The objective of the study was to test the viability of serum REST as a possible blood-based bio...
BACKGROUND: Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this stud...
Hidroxibutiratos/metabolismo,
Escherichia coli/metabolismo,
Escherichia coli/genética,
Eliminación de Gen,
Proteínas Represoras/genética,
Proteínas de Unión al ARN/genética,
Proteínas de Escherichia coli/genética,
Biopolímeros/genética,
Ligasas/metabolismo,
Proteínas Recombinantes,
Ingeniería Metabólica
ABSTRACT BACKGROUND: There is evidence that genetic predisposition and epigenetic alteration (e.g. DNA methylation) play major roles in lung cancer. In our genetic epidemiological studies, rs1970764 in oncogene PPP1R13L was most consistently associated with lung cancer risk. Here, we explored the role ...
MicroRNAs (miRNAs), as post-transcriptional regulators, have been reported to be involved in the initiation and progression of various types of cancer, including gastric cancer (GC). The present study aimed to investigate the role of miR-383-5p in gastric carcinogenesis. Cell viability was analyzed using...
Apoptosis,
Carcinogénesis/genética,
Carcinoma/genética,
Carcinoma/metabolismo,
Carcinoma/patología,
Proliferación Celular/genética,
Progresión de la Enfermedad,
Regulación hacia Abajo,
Regulación Neoplásica de la Expresión Génica,
Histona Desacetilasas/metabolismo,
MicroARNs/metabolismo,
Estadificación de Neoplasias,
ARN Mensajero/metabolismo,
Proteínas Represoras/metabolismo,
Neoplasias Gástricas/genética,
Neoplasias Gástricas/metabolismo,
Neoplasias Gástricas/patología
Batista, Fernando A;
Marcello, Marjory A;
Martins, Mariana B;
Peres, Karina C;
Cardoso, Ulieme O;
Silva, Aline C D N;
Bufalo, Natassia E;
Soares, Fernando A;
Silva, Márcio J da;
Assumpção, Lígia V;
Ward, Laura S.
ABSTRACT Objective The transcriptional repressor DREAM is involved in thyroid-specific gene expression, thyroid enlargement and nodular development, but its clinical utility is still uncertain. In this study we aimed to investigate whether DREAM mRNA levels differ in different thyroid tumors and how thi...
Biomarcadores de Tumor/genética,
Biomarcadores de Tumor/metabolismo,
Proteínas de Interacción con los Canales Kv/genética,
Proteínas de Interacción con los Canales Kv/metabolismo,
Estadificación de Neoplasias,
Pronóstico,
ARN Mensajero/genética,
ARN Mensajero/metabolismo,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Elementos Reguladores de la Transcripción/genética,
Proteínas Represoras/genética,
Proteínas Represoras/metabolismo,
Sensibilidad y Especificidad,
Neoplasias de la Tiroides/diagnóstico,
Neoplasias de la Tiroides/genética,
Neoplasias de la Tiroides/metabolismo
Introduction: Fetal hemoglobin is an important factor in modulating the severity of sickle cell anemia. Its level in peripheral blood underlies strong genetic determination. Associated loci with increased levels of fetal hemoglobin display population-specific allele frequencies. Objective: We investigate...
Anemia de Células Falciformes/genética,
Proteínas Portadoras/genética,
Etnicidad/genética,
Hemoglobina Fetal/genética,
Proteínas Nucleares/genética,
Polimorfismo de Nucleótido Simple,
Sitios de Carácter Cuantitativo/genética,
gamma-Globinas/genética,
Anemia de Células FalciformesSangre,
Anemia de Células Falciformes/etnología,
Población Negra/genética,
Colombia/epidemiología,
Genotipo,
Indígenas Sudamericanos/genética,
Polimorfismo de Longitud del Fragmento de Restricción,
Proteínas Represoras,
Senegal/etnología,
Sierra Leona/etnología
BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensiona...
Cromatina/fisiología,
Proteínas de Drosophila/fisiología,
Drosophila melanogaster/química,
Genoma de los Insectos/genética,
Mitosis/fisiología,
Proteínas Represoras/fisiología,
Ciclo Celular/fisiología,
Ensamble y Desensamble de Cromatina/fisiología,
Interfase/fisiología,
Secuencia de Bases,
Sitios de Unión,
Factor de Unión a CCCTC,
Biología Computacional,
Secuencia Conservada,
Conjuntos de Datos como Asunto,
Anotación de Secuencia Molecular,
Sintenía
