Em geral, a doença causada pelo vírus Zika (ZIKV) é clinicamente semelhante às de outros arbovírus. No entanto, ocasionalmente ela é associada a síndromes neurológicas como a síndrome de Guillain-Barré. Além disso, durante a gravidez a infecção pode causar a síndrome congênita do zika, com...
Avian coronavirus (AvCoV) infects a range of tissues in chickens and several other avian species. Although the virus can be isolated in chicken embryos, only a few strains of the 6 genotypes/33 lineages can grow in cell lines, with the Beaudette strain (GI-1 lineage) being the most used for in vitro stud...
Iani, Felipe Campos de Melo;
Caetano, Ana Carolina Barbosa;
Cocovich, Jéssica Caroline Wenceslau;
Amâncio, Frederico Figueiredo;
Pereira, Maira Alves;
Adelino, Talita Émile Ribeiro;
Caldas, Sérgio;
Silva, Marcos Vinícius Ferreira;
Pereira, Glauco de Carvalho;
Duarte, Myrian Morato.
BACKGROUND The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical v...
Anticuerpos Antivirales/sangre,
Antígenos Virales/sangre,
Antígenos Virales/inmunología,
Dengue/sangre,
Dengue/diagnóstico,
Dengue/virología,
Virus del Dengue/genética,
Virus del Dengue/inmunología,
Virus del Dengue/aislamiento & purificación,
Ensayo de Inmunoadsorción Enzimática/métodos,
Pruebas Inmunológicas/métodos,
Valor Predictivo de las Pruebas,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos,
Sensibilidad y Especificidad,
Proteínas no Estructurales Virales/genética,
Proteínas no Estructurales Virales/inmunología
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection depends on viral polyprotein processing, catalysed by the main proteinase (Mpro). The solution of the SARS-CoV-2 Mpro structure allowed the investigation of potential inhibitors. This work aims to provide first evidences of the applic...
BACKGROUND Since the World Health Organization (WHO) declared Coronavirus disease 2019 (COVID-19) to be a pandemic infection, important severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural proteins (nsp) have been analysed as promising targets in virtual screening approaches. Amon...
Betacoronavirus/efectos de los fármacos,
COVID-19,
Proteasas 3C de Coronavirus,
Infecciones por Coronavirus,
Cisteína Endopeptidasas,
Flavonoides/farmacología,
Glicósidos/farmacología,
Simulación del Acoplamiento Molecular,
Pandemias,
Neumonía Viral,
ARN Polimerasa Dependiente del ARN/antagonistas & inhibidores,
SARS-CoV-2,
Proteínas no Estructurales Virales/antagonistas & inhibidores
Objetivo: Se considera que el diagnóstico del dengue es fundamentalmente clínico; sin embargo, las pruebas rápidas basadas en la detección de IgM o NS1/IgM están siendo utilizadas en los servicios de salud. Este estudio determinó la contribución de las pruebas rápidas al diagnóstico de dengue e...
Abstract INTRODUCTION: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. METHODS: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-str...
Anticuerpos Antivirales/sangre,
Separación Celular,
Cromatografía de Afinidad,
Dengue/diagnóstico,
Virus del Dengue/genética,
Virus del Dengue/inmunología,
Citometría de Flujo,
Fluorescencia,
Leucocitos Mononucleares/virología,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Sensibilidad y Especificidad,
Proteínas no Estructurales Virales/genética
BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE ...
BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in Brazil. Because the clinical profile of patients affected by different DENV serotypes or ZIKV may be similar, the development of new methods...
Freire, Caio César de Melo;
Palmisano, Giuseppe;
Braconi, Carla T;
Cugola, Fernanda R;
Russo, Fabiele B;
Beltrão-Braga, Patricia CB;
Iamarino, Atila;
Lima Neto, Daniel Ferreira de;
Sall, Amadou Alpha;
Rosa-Fernandes, Livia;
Larsen, Martin R;
Zanotto, Paolo Marinho de Andrade.
BACKGROUND Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing s...
