Results: 27

Enzyme-linked immunosorbent assay (ELISA) for detection of IgY Anti-Borrelia anserina antibodies in Gallus gallus domesticus

Rev. bras. ciênc. vet; 29 (1), 2022
This study aimed to promote the standardization of an indirect, enzyme-linked immunosorbent assay (ELISA) for the serological detection of B. anserina in Gallus gallusdomesticus. An aliquoted sera from vaccinated chicken with B. anserina antigen (GI), experimental infected chickens with B. anserina (GII)...

Serological and molecular detection of Theileria equi in horses from Sinop, Mato Grosso state, Brazil

Rev. bras. ciênc. vet; 28 (3), 2021
Equine piroplasmosis is the most important tick-borne disease to affect horses in Brazil. Theileria equi is one of the causative agents of equine piroplasmosis. Chronic cases are expected, in which the animals show no apparent signs of infection and remain asymptomatic but constitute a source of the infe...

Inquérito sorológico de brucelose em machos e fêmeas bovinas em fazendas do Estado de Mato Grosso do Sul

Rev. bras. ciênc. vet; 28 (1), 2021
Objetivou-se avaliar a ocorrência de animais soro reagentes à brucelose bovina em fazendas localizadas no Estado de Mato Grosso do Sul, por meio de exame sorológico utilizando o Antígeno Acidificado Tamponado (AAT) e discutir as possíveis diferenças entre as soroprevalências de fêmeas e machos. F...

True prevalence and spatial distribution of equine infectious anemia virus (EIAV) in horses from northeast region of Brazil

Acta sci. vet. (Impr.); 49 (), 2021
Equine infectious anemia (EIA) is a viral infection, caused by a lentivirus of the Retroviridae family, Orthoretrovirinael subfamily and its occurrence generates significant economic losses due to culling of positive animals as a measure of infection control. The objective of this work was to determine t...

Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle

Pesqui. vet. bras; 40 (5), 2020
Serological techniques can detect antibodies against Sarcocystis spp., Neospora caninum and Toxoplasma gondii antigens in single or mixed infections. Immunofluorescent antibody tests (IFAT) is considered the gold standard technique for Sarcocystosis diagnostic in cattle serum and a positive IFAT result r...

Serological survey of bluetongue virus in sheep from Minas Gerais

Pesqui. vet. bras; 40 (4), 2020
Bluetongue is an infectious, non-contagious disease that affects domestic and wild ruminants, caused by a virus from the Orbivirus genus, Reoviridae family, transmitted by arthropod vectors of the Culicoides genus. This paper aims to be the first serological survey of bluetongue in sheep from the Meso-re...

High proportion of cattle and sheep seropositive and renal carriers of Leptospira sp. under semiarid conditions

Rev. bras. ciênc. vet; 27 (1), 2020
The aims of this study were to perform serological and molecular detection of Leptospira sp. infection in cattle and sheep under semiarid conditions. Based on a preliminary study performed in our research group, we selected six rural properties showing a positivity ≥ 60% for Sejroe serogroup with titer...

Evaluation of the performance of three serological tests for diagnosis of Leishmania infantum infection in dogs using latent class analysis

Abstract Canine leishmaniasis (CanL) is a disease caused by Leishmania infantum. Serological methods are the most common diagnostic techniques used for the diagnosis of the CanL. The objective of our study was to estimate the sensitivity and specificity of one in-house ELISA kit (ELISA UNIZAR) and three ...

Expression and antigenic analysis of the recombinant TRP36 protein from Ehrlichia canis São Paulo strain for serologic tests

Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiological pu...

Efficacy and limitations of different approaches to anticipate the diagnosis of pregnancy in cattle

The study evaluated sonographic and serologic exams performed for early (20 to 30d) diagnosis of pregnancy. One hundred-twenty (n= 120) bovine recipients were synchronized (estrous=D0) and timed embryo transferred (TET, D7) with fresh in vitro produced embryos. In the first trial (n= 46), diagnosis of pr...