Effects of chelating calcium in cryopreservation extender on frozen-thawed dog semen
Arq. bras. med. vet. zootec. (Online); 72 (6), 2020
We evaluated the effect of reducing free calcium in the cryopreservation medium, using the calcium chelator ethylene diamine tetracetic acid (EDTA) at 0.3% and 0.5% concentrations. Three male mixed breed dogs were subjected to semen collection by digital manipulation (n=16). Each ejaculate was divided in...