Results: 7

Overexpression of Chitinase A Gene from Serratia marcescens in Bacillus subtilis and Characterization of Enhanced Chitinolytic Activity

Abstract Chitinase enzymes possess various usages in agriculture, biotechnology and medicine due to their chitin degrading property. Thus, efficient production of chitinase enzymes with desired properties has importance for its use. In this study, chitinase A (chiA) gene from Serratia marcescens Bn10 was...

The potential of plant and fungal proteins in the control of gastrointestinal nematodes from animals

Abstract Gastrointestinal nematode infection is an important cause of high economic losses in livestock production. Nematode control based on a synthetic chemical approach is considered unsustainable due to the increasing incidence of anthelmintic resistance. Control alternatives such as the use of natur...

Transcriptome and proteome analyses of resistant preharvest peanut seed coat in response to Aspergillus flavus infection

BACKGROUND: The infection of peanut (Arachis hypogaea L.) seed coat by the pathogenic fungus Aspergillus flavus has highly negative economic and health impacts. However, the molecular mechanism underlying such defense response remains poorly understood. This study aims to address this issue by profiling ...

Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases

Mem. Inst. Oswaldo Cruz; 113 (2), 2018
BACKGROUND The insect chitinase gene family is composed by more than 10 paralogs, which can codify proteins with different domain structures. In Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil, a chitinase cDNA from adult female insects was previously characterized. The predict...

A broad pH range and processive chitinase from a metagenome library

Braz. j. med. biol. res; 50 (1), 2017
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading ...

Thermostable chitinase from Cohnella sp. A01: isolation and product optimization

Braz. j. microbiol; 47 (4), 2016
Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequen...

Actividad quitinolitica en extractos libres de celulas miceliales de Mucor rouxii
Chitinolytic activity in cell-free extracts from mycelial cells of Mucor rouxii

Rev. mex. micol; 3 (), 1987
Se detectó actividad de quitinasa en el citosol, en una fracción mixta de membranas y en la fracción de paredes celulares del micelio de M. rouxii. en todos los casos, la actividad quitinolítica fué mucho más efectiva contra quitina naciente que ccontra quitina preformada. Los resultados mostraron ...