LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Overexpression of Chitinase A Gene from Serratia marcescens in Bacillus subtilis and Characterization of Enhanced Chitinolytic Activity

Okay, Sezer; Alshehri, Wafa Abduallah.

Braz. arch. biol. technol; 63 (), 2020

Abstract Chitinase enzymes possess various usages in agriculture, biotechnology and medicine due to their chitin degrading property. Thus, efficient production of chitinase enzymes with desired properties has importance for its use. In this study, chitinase A (chiA) gene from Serratia marcescens Bn10 was...

Quitinasas/genética, Bacillus subtilis/enzimología, Serratia marcescens/enzimología, Expresión Génica, Temperatura, Concentración de Iones de Hidrógeno

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Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases

Ortigão-Farias, João Ramalho; Di-Blasi, Tatiana; Telleria, Erich Loza; Andorinho, Ana Carolina; Lemos-Silva, Thais; Ramalho-Ortigão, Marcelo; Tempone, Antônio Jorge; Traub-Csekö, Yara Maria.

Mem. Inst. Oswaldo Cruz; 113 (2), 2018

BACKGROUND The insect chitinase gene family is composed by more than 10 paralogs, which can codify proteins with different domain structures. In Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil, a chitinase cDNA from adult female insects was previously characterized. The predict...

Quitinasas/genética, Empalme Alternativo/genética, Quitinasas/fisiología, Sistema Digestivo/enzimología, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa

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A broad pH range and processive chitinase from a metagenome library

Thimoteo, SS; Glogauer, A; Faoro, H; de Souza, EM; Huergo, LF; Moerschbacher, BM; Pedrosa, FO.

Braz. j. med. biol. res; 50 (1), 2017

Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading ...

Quitina/química, Quitina/genética, Quitinasas/química, Quitinasas/genética, Cromatografía Líquida de Alta Presión, Escherichia coli, Expresión Génica/genética, Biblioteca de Genes, Vectores Genéticos, Concentración de Iones de Hidrógeno, Metagenoma/genética, Especificidad por Sustrato

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