LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Thermostable chitinase from Cohnella sp. A01: isolation and product optimization

Aliabadi, Nasrin; Aminzadeh, Saeed; Karkhane, Ali Asghar; Haghbeen, Kamahldin.

Braz. j. microbiol; 47 (4), 2016

Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequen...

Bacillus/metabolismo, Quitinasas/metabolismo, Bacillus/genética, Bacillus/aislamiento & purificación, Bacillus/ultraestructura, Carbono/metabolismo, Quitinasas/química, Activación Enzimática, Estabilidad de Enzimas/efectos de los fármacos, Concentración de Iones de Hidrógeno, Iones, Cinética, Metales, Nitrógeno/metabolismo, Fósforo/metabolismo, ARN Ribosómico 16S/genética, Análisis de Secuencia de ADN, Temperatura

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Chitinase from Enterobacter sp. NRG4: Its purification, characterization and reaction pattern

Enterobacter sp. NRG4 was shown to excrete chitinase into the culture supernatant when cultivated in medium containing chitin. A 60 kDa extracellular chitinase was purified to homogeneity and characterized. The enzyme hydrolyzed swollen chitin, colloidal chitin, regenerated chitin and glycol chitin but d...