Results: 6

Molecular diagnosis and intraspecific genetic variability of root pathogens of arid legumes in Western Rajasthan, India

Gautam, Ragini; Singh, S K; Sharma, Vinay.

Rev. biol. trop; 64 (4), 2016

Abstract:The productivity of arid legumes, such as Clusterbean (Cyamopsis tetragonoloba), Cowpea (Vigna unguiculata), Moth bean (Vigna aconitifolia) and Horse gram (Macrotyloma uniflorum), may remain stagnant over decades because of their high susceptibility to root diseases. Besides, there is a limitati...

ADN de Hongos, Fabaceae/genética, Fabaceae/microbiología, Fusarium/aislamiento & purificación, Variación Genética, Hypocreales/genética, Hypocreales/aislamiento & purificación, India, Técnicas de Diagnóstico Molecular/métodos, Mucorales/aislamiento & purificación, Enfermedades de las Plantas/microbiología, Raíces de Plantas/genética, Polimorfismo de Nucleótido Simple, Técnica del ADN Polimorfo Amplificado Aleatorio, Vigna/genética, Vigna/microbiología

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Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment

Kumar, Vinod; Sharma, Ashwani; Narasimha Prasad, Bellur Chayapathy; Bhaskar Gururaj, Harishchandra; Aswathanarayana Ravishankar, Gokare.

Electron. j. biotechnol; 9 (4), 2006

The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance transforma...

Agrobacterium/genética, Plantas/genética, Plantas/microbiología, Raíces de Plantas/genética, Sonicación, Transformación Genética, Acetofenonas, ADN Bacteriano/genética, Cloruro de Calcio, Reacción en Cadena de la Polimerasa

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Analysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae)

Cardillo, Alejandra B; Giulietti, Ana M; Marconi, Patricia L.

Electron. j. biotechnol; 9 (3), 2006

Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologically- important group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-â hydroxylase (H6H, EC 1.14.11.11) at the end of ...

Brugmansia/genética, Raíces de Plantas/genética, Solanaceae/genética, Análisis de Secuencia de ARN, Transformación Genética, Secuencia de Bases, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa, Bases de Datos de Ácidos Nucleicos, Clonación Molecular, Oxigenasas de Función Mixta/genética, Brugmansia/enzimología, Solanaceae/enzimología, Raíces de Plantas/enzimología, ADN Complementario, Biología Computacional, Escopolamina/metabolismo, Oxigenasas de Función Mixta/metabolismo

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Rooting in Km selective media as efficient in vitro selection method for sunflower genetic transformation

Radonic, Laura Mabel; Zimmermann, Julián Marcos; Zavallo, Diego; López, Nilda; López Bilbao, Marisa.

Electron. j. biotechnol; 9 (3), 2006

Despite of numerous publications in sunflower genetic transformation, there is no efficient or reproducible protocol with low number of escapes. The latter would indicate that the selection method is not effective. In this work we used Km as selective agent, Agrobacterium tumefaciens EHA105 strain and a ...

Helianthus/genética, Transformación Genética, Agrobacterium tumefaciens, Técnicas In Vitro, Raíces de Plantas/genética, Reacción en Cadena de la Polimerasa, Coloración y Etiquetado, Kanamicina/administración & dosificación, Antibacterianos/administración & dosificación, Transgenes, Color, Glucuronidasa, Regeneración

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A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database

Jayashree, B; Buhariwalla, Hutokshi K; Shinde, Sanjeev; Crouch, Jonathan H.

Electron. j. biotechnol; 8 (2), 2005

Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Publ...

Cicer/genética, Raíces de Plantas/genética, Fabaceae, Resistencia a la Sequía/genética, Genómica, Etiquetas de Secuencia Expresada, Medicago truncatula, Biología Computacional, Minería de Datos, Clonación Molecular, Hibridación Genética, Genotipo, Estrés Fisiológico

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Peroxidase production from hairy root cultures of red beet (Beta vulgaris)

Rudrappa, Thimmaraju; Neelwarne, Bhagyalakshmi; Kumar, Vinod; Lakshmanan, Venkatachalam; Venkataramareddy, Sreedhar Reddampalli; Aswathanarayana, Ravishankar Gokare.

Electron. j. biotechnol; 8 (2), 2005

The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, t...

Beta vulgaris/enzimología, Peroxidasa/biosíntesis, Agrobacterium/genética, Beta vulgaris/genética, Plantas Modificadas Genéticamente/enzimología, Hormona del Crecimiento, Biomasa, Raíces de Plantas/genética, Clonación Molecular, Reacción en Cadena de la Polimerasa, Concentración de Iones de Hidrógeno, ADN Bacteriano, Transformación Genética, Raíces de Plantas/enzimología, Ácidos Indolacéticos

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