LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Media development and process parameter optimization using statistical experimental designs for the production of nonribosomal peptides in Escherichia coli

Oestreich, Arne Michael; Ilire Suli, Merlinda; Gerlach, Doreen; Fan, Rong; Czermak, Peter.

Electron. j. biotechnol; 52 (), 2021

BACKGROUND: Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and a promising candidate for the synthe...

Escherichia coli, Biotecnología, Péptido Sintasas/metabolismo, Bioingeniería, Modelos Estadísticos, Reactores Biológicos/microbiología, Electroforesis en Gel de Poliacrilamida, Temperatura, Concentración de Iones de Hidrógeno, Carbono/metabolismo

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Ammonia determines transcriptional profile of microorganisms in anaerobic digestion

ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscripto...