LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

A duplex probe-directed recombinase amplification assay for detection of single nucleotide polymorphisms on 8q24 associated with prostate cancer

Single nucleotide polymorphisms (SNPs) have important application value in the research of population genetics, hereditary diseases, tumors, and drug development. Conventional methods for detecting SNPs are typically based on PCR or DNA sequencing, which is time-consuming, costly, and requires complex in...

Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics

Sundarrajan, Sudarson; Rao, Sneha; Padmanabhan, Sriram.

Braz. j. microbiol; 49 (4), 2018

ABSTRACT We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was p...

Proteínas Bacterianas/genética, Proteínas Bacterianas/aislamiento & purificación, Proteínas Bacterianas/metabolismo, Clonación Molecular, Escherichia coli/genética, Escherichia coli/metabolismo, Expresión Génica, Virus de la Hepatitis B/genética, Virus de la Hepatitis B/aislamiento & purificación, Reacción en Cadena de la Polimerasa/instrumentación, Reacción en Cadena de la Polimerasa/métodos, Recombinasas/genética, Recombinasas/aislamiento & purificación, Recombinasas/metabolismo, Thermus thermophilus/enzimología, Thermus thermophilus/genética

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