Results: 17

Standardization and performance evaluation of a virus isolation technique using murine neuroblastoma cell line (N2A) for the rabies virus (RABV) surveillance in Pará State, Brazil

Rev. bras. ciênc. vet; 30 (3), 2023
Rabies is a zoonotic disease caused by the rabies virus (RABV) from the Lyssavirus rabies species. In humans, around 60,000 deaths are registered annually in the world due to the disease, while accurate estimation of rabies incidence among other animals, mainly in wildlife, is unfeasible. In recent decad...

Aislación y caracterización de las células epiteliales del amnios

Int. j. morphol; 40 (3), 2022
RESUMEN: Las células epiteliales del amnios (hAECs) son células madre pluripotenciales; tienen capacidad de diferenciarse en células de las tres capas embrionarias. Como tales, se utilizan en algunas terapias regenerativas en medicina. Este estudio tiene por objetivo describir un protocolo de aislaci�...

Optimizing the recovery of peripheral blood mononuclear cells trapped in leukoreduction filters - A comparison study

Abstract Introduction The isolation of captured peripheral blood mononuclear cells (PBMNCs) from leukoreduction filters (LRFs) can be of great importance in terms of bringing the lost cells back into use. Objective The aim of this study was to evaluate various methods based on their potential to recove...

Identification of new human mesenchymal stem cell biomarker by aptamers

Stem cells are undifferentiated cells that can be distinguished from others by their ability to self-renew and to differentiate into new specific cell types. Mesenchymal stem cells (MSC) are adult stem cells that can be obtained from different sources, such as adipose tissue, bone marrow, dental pulp, an...

Papila Apical Dentaria como fuente de Células Madre: Relato del Caso

Las células madre mesenquimales (MSCs) son células madre adultas que tienen la capacidad de diferenciarse en varios tipos de células. Una rica fuente de células madre mesenquimales puede ser obtenida de tejidos dentales como la papila apical. El objetivo de este trabajo fue extraer las células madre...

Establecimiento e implementación de un protocolo simplificado de expansión y cultivo de Células Madre de Pulpa Dental Humana (DPSCh)

Odontoestomatol; 23 (38), 2021
Resumen Objetivos: Establecer e implementar un protocolo simplificado de extracción, aislamiento primario y cultivo de células madre derivadas de la pulpa dental humana (DPSCh). Analizar cuantitativamente y cualitativamente las células aisladas. Metodología: 10 terceros molares sanos donados por ...

Impact of cell harvesting methods on detection of cell surface proteins and apoptotic markers

Braz. j. med. biol. res; 54 (2), 2021
Assays based on the flow cytometry technique allow a convenient analysis of multiple cellular parameters; however, their results should be interpreted cautiously due to a strong impact of confounding factors. Different techniques in cell culturing such as either enzymatic or mechanic detachment of adhere...

Comparison of protein precipitation methods for sample preparation prior to proteomic analysis of Chinese hamster ovary cell homogenates

BACKGROUND: Chinese hamster ovary (CHO) cells are the workhorse for obtaining recombinant proteins. Proteomic studies of these cells intend to understand cell biology and obtain more productive and robust cell lines for therapeutic protein production in the pharmaceutical industry. Because of the great i...

Procedimiento para el cultivo e identificación de células madre obtenidas de lipoaspirado humano con fines de investigación

RESUMEN Las células madre humanas nacen con la creación de la vida misma y algunas de estas permanecen durante toda la vida. Por consiguiente, se pueden hallar en tejidos adultos y utilizarlas para investigaciones a nivel básico y aplicado. Actualmente, en nuestro país existe un creciente interés en...

Phenotype changes of oral epithelial stem cells after in vitro culture

Abstract The aim of our study was to isolate populations of keratinocyte stem cells based on the expression of cell surface markers and to investigate whether the culture could affect their phenotype. keratinocytes from human oral mucosa were sorted based on the expression of the epithelial stem cell mar...