Results: 2

Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene

Braz. j. microbiol; 49 (2), 2018
Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was...

Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR

Braz. j. microbiol; 47 (2), 2016
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samp...