LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Protocol for chromosome-specific probe construction using PRINS, micromanipulation and DOP-PCR techniques

PASSAMANI, PAULO Z; CARVALHO, CARLOS R; SOARES, FERNANDA A F.

An. acad. bras. ciênc; 90 (1), 2018

ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate ol...

Cromosomas Humanos X/genética, Análisis Citogenético/métodos, Cartilla de ADN/genética, Sondas de ADN/genética, Hibridación Fluorescente in Situ/métodos, Microdisección/métodos, Reacción en Cadena de la Polimerasa/métodos, Etiquetado in Situ Primed/métodos, Reproducibilidad de los Resultados

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