BACKGROUND: Piercing/sucking insect pests in the order Hemiptera causes substantial crop losses by removing photoassimilates and transmitting viruses to their host plants. Cloning and heterologous expression of plantderived insect resistance genes is a promising approach to control aphids and other sap-s...
Pinellia/química,
Control Biológico de Vectores,
Áfidos/patogenicidad,
Hojas de la Planta/química,
Resistencia a la Enfermedad,
Virus de Plantas,
Regiones Promotoras Genéticas,
Plantas Modificadas Genéticamente,
Nicotiana,
Protección de Cultivos,
Reacción en Cadena de la Polimerasa,
Southern Blotting,
Transgenes
Background: Hydrophobins are small proteins secreted by filamentous fungi, which show a highly surface activity. Because of the signally self-assembling abilities and surface activities, hydrophobins were considered as candidates in many aspects, for example, stabilizing foams and emulsions in food produ...
Proteínas Fúngicas/genética,
Proteínas Fúngicas/metabolismo,
Clonación Molecular,
Lentinula/genética,
Lentinula/metabolismo,
Basidiomycota/metabolismo,
Emulsionantes,
Levaduras,
Agrobacterium tumefaciens/metabolismo,
Southern Blotting,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo,
Electroforesis en Gel de Poliacrilamida,
Basidiomycota/enzimología,
Transformación Genética,
Microscopía Fluorescente,
Análisis de Secuencia
Abstract Introduction: The FMR1 gene has four allelic variants according to the number of repeats of the CGG triplet. Premutation carriers with between 55 and 200 repeats are susceptible to developing pathologies such as tremor and ataxia syndrome (FXTAS) and fragile X-associated primary ovarian insuff...
Liu, Juhua;
Gao, Pengzhao;
Sun, Xiuxiu;
Zhang, Jing;
Sun, Peiguang;
Wang, Jiashui;
Jia, Caihong;
Zhang, Jianbin;
Hu, Wei;
Xu, Biyu;
Jin, Zhiqiang.
Background: Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Conventional breeding has been seriously hampered by their long generation time, polyploidy, and sterility of most cultivated varieties. Establishment of an efficient regeneration and transformatio...
Musa/crecimiento & desarrollo,
Musa/genética,
Transformación Genética,
Plantas Modificadas Genéticamente,
Regeneración,
Agrobacterium tumefaciens/fisiología,
Musa/microbiología,
Organogénesis de las Plantas,
Reacción en Cadena de la Polimerasa,
Southern Blotting,
Inmunohistoquímica,
Glucuronidasa
Background The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidas...
Poligalacturonasa/metabolismo,
Poligalacturonasa/genética,
Peroxidasa/metabolismo,
Peroxidasa/genética,
Cloroplastos/genética,
Nicotiana,
Hongos/enzimología,
Bacterias/enzimología,
Pared Celular,
Transformación Genética,
Hidrolasas,
Reacción en Cadena de la Polimerasa,
Temperatura,
Concentración de Iones de Hidrógeno,
Southern Blotting
Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pat...
Jatropha/enzimología,
Isomerasas de Doble Vínculo Carbono-Carbono/metabolismo,
Isomerasas de Doble Vínculo Carbono-Carbono/genética,
Hemiterpenos/metabolismo,
Hemiterpenos/genética,
Southern Blotting,
Clonación Molecular,
Jatropha/química,
ADN Complementario/síntesis química,
ARN/aislamiento & purificación,
Filogenia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Expresión Génica,
Cloroplastos
A infecçäo de gatos domésticos pelo Vírus da Imunodeficiência Felina (FIV) é um dos modelos mais promissores para o estudo da infecçäo pelo vírus da imunodeficiência humana (HIV) que causa a Síndrome de Imunodeficiência Adquirida (AIDS). O FIV causa, em gatos, uma enfermidade similar àquela ...
A superóxido dismutase (SOD) tem um papel protetor contra toxicidade do oxigênio por remover radicais superóxido (`O IND. 2 POT. -ï) através da sua dismutação para `H IND. 2'`O IND. 2'. Células de levedura apresentam duas SODs intracelulares, uma contendo Cu e Zn e outra Mn em seus sítios ativos...
Tem sido relatado que mulheres infectadas pelo HIV apresentam maior incidência de atipias citológicas nos esfregaços cérvico-vaginais, relacionadas principalmente com a infecçao pelo HPV. MATERIAL E MÉTODOS: O presente trabalho apresenta o estudo de esfregaços cérvico-vaginais de 147 mulheres HIV...
