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High-level soluble expression of phospholipase D from Streptomyces chromofuscus in Escherichia coli by combinatorial optimization

Wu, Rong; Cao, Jun; Liu, Feixiang; Yang, Meng; Su, Erzheng.
Electron. j. biotechnol; 50 (), 2021
BACKGROUND: Phospholipase D (PLD) is used as the biocatalyst for phosphatidylserine (PS) production. In general, PLD was expressed in insoluble form in Escherichia coli. High-level soluble expression of PLD with high activity in E. coli is very important for industrial production of PLD. RESULTS: Strepto...
Streptomyces/enzimología, Fosfolipasas/metabolismo, Escherichia coli, Técnicas Químicas Combinatorias, Solubilidad, Streptomyces/genética, Temperatura, Codón
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A Thermotolerant Xylan-Degrading Enzyme Is Produced by Streptomyces malaysiensis AMT-3 Using by-Products From the Food Industry

Nascimento, Rodrigo Pires do; Reis, Alberto Delgado; Gírio, Francisco; Pereira Jr, Nei; Bon, Elba Pinto da Silva; Coelho, Rosalie Reed Rodrigues.
Braz. arch. biol. technol; 63 (), 2020
Abstract This study evaluated the production of endoxylanases by Streptomyces malaysiensis AMT-3 in submerged fermentation using by-products of the food industry at 28ºC. In shake-flasks experiments, the highest endoxylanase activity of 45.8 U.mL-1 was observed within 6 days in a medium containing (w/v)...
Industria de Alimentos, Streptomyces/enzimología, Temperatura, Endo-1,4-beta Xilanasas/biosíntesis, Fermentación
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Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens

M.M. Silva, Germana; Bezerra, Raquel P; Teixeira, José A; Porto, Tatiana S; Lima-Filho, José L; Porto, Ana Lúcia F.
Electron. j. biotechnol; 18 (1), 2015
Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal c...
Péptido Hidrolasas/biosíntesis, Fibrinolíticos/metabolismo, Streptomyces/enzimología, Actinobacteria, Glucosa/análisis, Líquenes, Glycine max, Harina, Modelos Estadísticos
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