Results: 25

Caracterización clínica y microbiológica de pacientes con trazas en Xpert MTB/RIF Ultra

INTRODUCCIÓN: El Xpert MTB/RIF Ultra (Ultra) ha mejorado dramáticamente el diagnóstico de la tuberculosis (TBC). Con él ha nacido la categoría de trazas, que es la menor carga bacilar detectable por este examen. OBJETIVO: Describir las características clínicas de los pacientes con presencia de tra...

Demanda de culturas microbiológicas e prevalência de microrganismos em um hospital universitário de Pernambuco

Objetivo: verificar a demanda de hemoculturas, aspirados traqueais e uroculturas realizadas no HU-UNIVASF/ EBSERH e a prevalência dos microrganismos identificados no período de janeiro a junho de 2016. Métodos: estudo retrospectivo documental com abordagem quantitativa. Resultados: o setor de microbio...

Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

Braz. j. biol; 79 (4), 2019
Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacte...

Validation of multiplex PCR for the diagnosis of acute bacterial meningitis in culture negative cerebrospinal fluid

Arq. neuropsiquiatr; 77 (4), 2019
ABSTRACT This study evaluated the operational characteristics of the multiplex polymerase chain reaction (PCR) for cerebrospinal fluid (CSF) from patients with cellular and biochemical characteristics of acute bacterial meningitis and positive or negative CSF cultures. Methods: Multiplex PCR was perform...

Increase in the diagnosis of mycobacteremia after the implementation of semi-automated blood culture at a Brazilian public health laboratory

Abstract INTRODUCTION The prevalence of hematogenous dissemination of mycobacteria is high in immunosuppressed patients. The isolation of mycobacteria in culture remains the standard procedure. METHODS This is a cross-sectional study based on the results of solid (Löwenstein-Jensen medium) and semi-...

Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR

Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. R...

Relationship between bacilloscopy and operational classification of Hansen's disease in patients with reactions

An. bras. dermatol; 93 (3), 2018
Abstract: Leprosy can be classified according to its operational form as paucibacillary or multibacillary. Bacilloscopy integrates its diagnostic armamentarium. Patients with the disease may present leprosy reactions. This study describes the association of bacilloscopy results and the type of operationa...

Identificación de micobacterias en medio sólido mediante microscopía de fase invertida y tinción Ziehl-Neelsen

RESUMEN Con el objetivo de identificar diferencias morfológicas en colonias de diferentes especies de micobacterias mediante microscopía de fase invertida, se sembraron en medio sólido 7H11, nueve especies de micobacterias patógenas de alta prevalencia y se observaron hasta por 21 días. Adicionalmen...

Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species

Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RES...