Um dos maiores desafios no desenvolvimento de produtos probióticos é entender como os microrganismos interagem entre si e com o hospedeiro. Quando falamos em alimentos fermentados tradicionais, este obstáculo aumenta porque a matriz alimentar já possui um microbioma intrínseco. No entanto, também ...
RESUMEN Objetivo. Evaluar el crecimiento y la supervivencia de Cryphiops caementarius en cocultivo con Oreochromis niloticus a diferentes densidades. Materiales y métodos. Se utilizaron camarones machos (5.86 cm y 7.65 g) y alevines revertidos de tilapia (5.65 cm y 2.61 g). Se emplearon nueve acuario...
BACKGROUND: Opsonization, is the molecular mechanism by which target molecules promote interactions with phagocyte cell surface receptors to remove unwanted cells by induced phagocytosis. We designed an in vitro system to demonstrate that this procedure could be driven to eliminate adipocytes, using pept...
BACKGROUND: The preparation of broad bean koji is a key process in the production of Pixian broad bean paste (PBP). Protease is essential for the degradation of proteins during PBP fermentation. To obtain broad bean koji with high protease activity using the cocultivated strains of Aspergillus oryzae QM-...
In vitro modeling of neurodegenerative diseases is now possible by using patient-derived induced pluripotent stem cells (iPS). Through them, it is nowadays conceivable to obtain human neurons and glia, and study diseases cellular and molecular mechanisms, an attribute that was previously unavailable to a...
Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultu...
Ascomicetos/crecimiento & desarrollo,
Ascomicetos/aislamiento & purificación,
Ascomicetos/metabolismo,
Técnicas de Cocultivo,
Endófitos/crecimiento & desarrollo,
Endófitos/aislamiento & purificación,
Endófitos/metabolismo,
Fitoquímicos/metabolismo,
Rumex/crecimiento & desarrollo,
Rumex/metabolismo,
Rumex/microbiología,
Plantones/crecimiento & desarrollo,
Plantones/metabolismo,
Plantones/microbiología,
Factores de Tiempo
However, folate production was strain-dependent and also dependent on the environmental conditions and on the vegetable substrate used. Passion fruit by-product presented the lowest folate concentration and was selected for the following experiments. Thus, the impact of the supplementation of soymilk wit...
Ácidos Pteroilpoliglutámicos/antagonistas & inhibidores,
Frutas/efectos adversos,
Plantas/efectos adversos,
Glycine max/efectos adversos,
Alimentos Funcionales/análisis,
Antiinflamatorios/farmacología,
Leche de Soja/farmacología,
Prebióticos/análisis,
Probióticos/análisis,
Técnicas In Vitro/métodos,
Técnicas de Cocultivo/métodos
Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages o...
Abstract Osteonecrosis of the jaw is an adverse effect of bisphosphonates. While the etiopathogenesis of this condition has been investigated, the interactions and effects of bisphosphonates on oral mucosa cells remain unclear. It is hypothesized that cell culture models, such as co-culture or three-dime...
Análisis de Varianza,
Conservadores de la Densidad Ósea/farmacología,
Técnicas de Cultivo de Célula/métodos,
Proliferación Celular/efectos de los fármacos,
Supervivencia Celular/efectos de los fármacos,
Células Cultivadas,
Técnicas de Cocultivo,
Difosfonatos/farmacología,
Ensayo de Inmunoadsorción Enzimática,
Células Epiteliales/efectos de los fármacos,
Fibroblastos/efectos de los fármacos,
Encía/citología,
Imidazoles/farmacología,
Reproducibilidad de los Resultados,
Estadísticas no Paramétricas,
Factores de Tiempo,
Ácido Zoledrónico
BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocyte...
Células Epiteliales/efectos de los fármacos,
Hormona del Crecimiento/farmacología,
Laminina/biosíntesis,
Timocitos/efectos de los fármacos,
Timo/citología,
Adhesión Celular/efectos de los fármacos,
Diferenciación Celular/efectos de los fármacos,
Movimiento Celular/efectos de los fármacos,
Citometría de Flujo/métodos,
Integrina alfa6beta1/análisis,
Integrina alfa6beta1/metabolismo,
Laminina/efectos de los fármacos,
Timo/metabolismo,
Análisis de Varianza,
Linfocitos T CD4-Positivos,
Linfocitos T CD8-positivos,
Células Cultivadas,
Técnicas de Cocultivo,
Inmunohistoquímica,
Valores de Referencia,
Factores de Tiempo
