Salazar, S;
Gutiérrez, N;
Sánchez, O;
Ramos, E;
González, A;
Acosta, J;
Ramos, T;
Altamirano, C;
Toledo, JR;
Montesino, R.
BACKGROUND: Lawsonia intracellularis remains a problem for the swine industry worldwide. Previously, we designed and obtained a vaccine candidate against this pathogen based on the chimeric proteins: OMP1c, OMP2c, and INVASc. These proteins formed inclusion bodies when expressed in E. coli, which induced...
Lawsonia (Bacteria)/inmunología,
Infecciones por Desulfovibrionaceae/prevención & control,
Vacunas Bacterianas/inmunología,
Vacunas Bacterianas/administración & dosificación,
Vacunación,
Inmunidad,
Técnicas de Cultivo Celular por Lotes,
Fermentación,
Vacunas Sintéticas,
Enfermedades de los Porcinos/inmunología,
Enfermedades de los Porcinos/prevención & control,
Porcinos,
Supervivencia Celular
Among the methods described for determining the solubility, shake-flask is suitable to evaluate the equilibrium solubility according to the BCS. Nevertheless, experimental conditions related to the shake-flask method are not well described. Evaluating the effects of experimental conditions on solubility ...
Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines. Multiple copies of integrated genes by retrovector transduction res...
BACKGROUND: Eugenol is an economically favorable substrate for the microbial biotransformation of aromatic compounds. Coniferyl aldehyde is one kind of aromatic compound that is widely used in condiment and medical industries; it is also an important raw material for producing other valuable products suc...
Abstract Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the a...
Abstract Pullulan is a natural exopolysaccharide with many useful characteristics. However, pullulan is more costly than other exopolysaccharides, which limits its effective application. The purpose of this study was to adopt a novel mixed-sugar strategy for maximizing pullulan production, mainly using p...
Almidón/metabolismo,
Glucanos/biosíntesis,
Fermentación,
Ascomicetos/metabolismo,
Sacarosa/metabolismo,
Técnicas de Cultivo Celular por Lotes,
Biomasa,
Reactores Biológicos,
Carbono/metabolismo,
Cinética,
Almidón/química,
Solanum tuberosum/química
ABSTRACT Lactic acid, which can be obtained through fermentation, is an interesting compound because it can be utilized in different fields, such as in the food, pharmaceutical and chemical industries as a bio-based molecule for bio-refinery. In addition, lactic acid has recently gained more interest due...
The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the e...
Background: A thermostable lipase MAS1 from marine Streptomyces sp. strain was considered as a potential biocatalyst for industrial application, but its production level was relatively low. Here, the effect of chaperones co-expression on the secretory expression of lipase MAS1 in Pichia pastoris was inve...
Lipasa/biosíntesis,
Streptomyces/metabolismo,
Pichia,
Fermentación,
Proteína Disulfuro Isomerasas,
Vitreoscilla,
Técnicas de Cultivo Celular por Lotes,
Chaperonas Moleculares,
Supervivencia Celular,
Reactores Biológicos,
Temperatura,
Concentración de Iones de Hidrógeno
Background In the field of microbial fermentation technology, how to optimize the fermentation conditions is of great crucial for practical applications. Here, we use artificial neural networks (ANNs) and support vector machine (SVM) to offer a series of effective optimization methods for the production ...
