The luciferase reporter system of the MMP12 endogenous promoter for investigating transcriptional regulation of the human MMP12 gene
Electron. j. biotechnol; 43 (), 2020
Background: Matrix metalloproteinase 12 (MMP12), a member of MMPs, can take lots of roles including extracellular matrix component degradation, viral infection, inflammation, tissue remodeling and tumorigenesis. To explore the transcriptional regulation of MMP12 gene, a sensitive luciferase reporter HEK2...
Luciferasas/genética, Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas, Metaloproteinasa 12 de la Matriz/genética, Transcripción Genética, Matriz Extracelular, Comunicación Celular, Línea Celular, Técnicas de Cultivo de Célula, Regiones Promotoras Genéticas/genética, Sistemas CRISPR-Cas, Técnicas de Sustitución del Gen