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Intervenciones genéticas de Aedes aegypti para el control de arbovirosis

Norma Antonio, Michelle; Traverso Huarcaya, Marco Fabriccio; Traverso Castillo, Camila Alejandra; Castillo Rodriguez, María Nelly.
Bol. malariol. salud ambient; 62 (4), 2022
La ineficacia de las estrategias actuales para el control químico de los mosquitos vectores plantea la necesidad de desarrollar enfoques novedosos, entre estos están las estrategias genéticas para reducir las poblaciones de mosquitos vectores o sustituirlos por aquellos que no son capaces de transmiti...
Aedes, Arbovirus, Genética, Ingeniería Genética, Técnicas de Transferencia de Gen, Mosquitos Vectores, Infecciones por Arbovirus, Estrategias de Salud
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Lentivirus vectors fail to deliver transgenes into bovine zygotes after co-incubation with sperm during in vitro fertilization

Lemos, D. R; Souza, G. T; Souza, V. G. P; Ribeiro, L. S; Saraiva, N. Z; Quintão, C. C. R; Camargo, L. S. A.
Arq. bras. med. vet. zootec. (Online); 73 (1), 2021
As vantagens dos animais transgênicos têm sido demonstradas em diferentes aplicações, entretanto muitas metodologias usadas para gerar animais geneticamente modificados (GM) apresentam baixas taxas de eficiência. O objetivo deste estudo foi avaliar a entrega dos vetores lentivirais (VLs) em zigotos ...
Animales Modificados Genéticamente/genética, Transgenes, Embrión de Mamíferos, Cigoto, Técnicas de Transferencia de Gen/veterinaria, Vectores Genéticos/análisis, Fertilización In Vitro/veterinaria
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Learning from transgenics: advanced gene editing technologies should also bridge the gap with traditional genetic selection

Arencibia, Ariel D; D'Afonseca, Vívian; Chakravarthi, Mohan; Castiglione, Stefano.
Electron. j. biotechnol; 41 (), 2019
We highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independe...
Técnicas de Transferencia de Gen, Edición Génica, Sistemas CRISPR-Cas, Epigénesis Genética, Mutagénesis, Metilación de ADN, Mejoramiento Genético, Transformación Genética, Plantas Modificadas Genéticamente
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An update on the tools for creating transgenic animal models of human diseases - focus on atherosclerosis

Volobueva, A S; Orekhov, A N; Deykin, A V.
Braz. j. med. biol. res; 52 (5), 2019
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disea...
Animales Modificados Genéticamente, Aterosclerosis/genética, Aterosclerosis/fisiopatología, Investigación Biomédica/métodos, Modelos Animales de Enfermedad, Técnicas de Transferencia de Gen, Ingeniería Genética/métodos, Nucleasas de los Efectores Tipo Activadores de la Transcripción/metabolismo
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Perspectives for cancer immunotherapy mediated by p19Arf plus interferon-beta gene transfer

Strauss, Bryan E; Silva, Gissele Rolemberg Oliveira; de Luna Vieira, Igor; Cerqueira, Otto Luiz Dutra; Del Valle, Paulo Roberto; Medrano, Ruan Felipe Vieira; Mendonça, Samir Andrade.
Clinics; 73 (supl.1), 2018
While cancer immunotherapy has gained much deserved attention in recent years, many areas regarding the optimization of such modalities remain unexplored, including the development of novel approaches and the strategic combination of therapies that target multiple aspects of the cancer-immunity cycle. Ou...
Muerte Celular/genética, Inhibidor p16 de la Quinasa Dependiente de Ciclina/genética, Técnicas de Transferencia de Gen, Terapia Genética/métodos, Inmunoterapia/métodos, Interferón beta/uso terapéutico, Neoplasias/inmunología, Neoplasias/terapia, Sistemas de Lectura/genética, Proteína p14ARF Supresora de Tumor/genética
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Introduction of a synthetic Thermococcus-derived α-amlyase gene into barley genome for increased enzyme thermostability in grains

Mihálik, Daniel; Gubišová, Marcela; Kraic, Ján; Hudcovicová, Martina; Havrlentová, Michaela; Moravčíková, Jana; Glasa, Miroslav; Matušíková, Ildikó.
Electron. j. biotechnol; 30 (), 2017
Background: The enzymes utilized in the process of beer production are generally sensitive to higher temperatures. About 60% of them are deactivated in drying the malt that limits the utilization of starting material in the fermentation process. Gene transfer from thermophilic bacteria is a promising too...
Thermococcus/metabolismo, alfa-Amilasas/metabolismo, Cerveza, alfa-Amilasas/genética, Termotolerancia, Clonación Molecular, Estabilidad de Enzimas, Concentración de Iones de Hidrógeno, Hordeum/enzimología, Hordeum/genética, Técnicas de Transferencia de Gen, Fermentación, Plantas Modificadas Genéticamente/enzimología, Transformación Genética, Semillas/enzimología, Semillas/genética, Calor, Hordeum/microbiología, Semillas/microbiología
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Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro

Pan, Zeya; Pan, Hao; Zhang, Jin; Yang, Yun; Liu, Hui; Yang, Yuan; Huang, Gang; Ni, Junsheng; Huang, Jian; Zhou, Weiping.
Biol. Res; 48 (), 2015
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcino...
Carcinoma Hepatocelular/patología, Ciclo Celular, Proliferación Celular, Lentivirus/genética, Neoplasias Hepáticas/patología, Proteínas de Neoplasias/metabolismo, Interferencia de ARN/fisiología, Proteasas Ubiquitina-Específicas/metabolismo, Apoptosis, Western Blotting, Carcinoma Hepatocelular/enzimología, Ciclo Celular/genética, Línea Celular Tumoral, Proliferación Celular/genética, Regulación Neoplásica de la Expresión Génica/genética, Técnicas de Silenciamiento del Gen, Silenciador del Gen, Técnicas de Transferencia de Gen, Técnicas In Vitro, Neoplasias Hepáticas/enzimología, Proteínas de Neoplasias/genética, Reacción en Cadena en Tiempo Real de la Polimerasa, Proteasas Ubiquitina-Específicas/genética
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Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line

Crispo, Martina; Schlapp, Geraldine; Cárdenas-Rodríguez, Magdalena; González-Maciel, Dolores; Rumbo, Martín.
Electron. j. biotechnol; 16 (6), 2013
Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize ...
Técnicas de Transferencia de Gen, Luciferasas/genética, Quimiocina CXCL2, ADN/análisis, Regiones Promotoras Genéticas, Clonación Molecular, Técnicas de Cultivo de Célula, Técnicas In Vitro, Transferencia de Embrión, Genotipo, Microinyecciones
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