Results: 3

Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics

Braz. j. microbiol; 49 (4), 2018
ABSTRACT We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was p...

Hydrogen/rydride ion relay - a mechanism for early electron transfer in cytochrome c oxidases

West Indian med. j; 62 (1), 2013
Cytochrome c oxidase (COX) employs electrons obtained from cytochrome c to bring about the reduction of oxygen to water. It is known that the electrons originate from the haem edge of cytochrome c and enters bovine COX at Trp-104. It is also known that Tyr-105, Glu-198 and Asp-158 of COX subunit II play ...

Atenuación térmica de cultivos lácticos destinados a la maduración acelerada de quesos
Attenuation thermophilus of lactic cultivation destinated maturation acelerated of cheese

En el presente trabajo se evalúa el efecto de la atenuación mediante choque térmico a varias temperaturas de cultivos de streptococcus salivarius ssp. thermophilus y lactobacillus delbrueckii ssp. bulgaricus destinados a la maduración acelerada de queso tipo Dambo. El objetivo fue establecer condicio...