Drought is one of the major constraints in durum wheat production in the Mediterranean Basin. In order to overcome this problem, the genetic transformation of durum wheat is one of the choices for improvement. However, the recalcitrance to Agrobacterium-mediated transformation in durum wheat (Triticum tu...
Chloroplast biotechnology has emerged as a promissory platform for the development of modified plants to express products aimed mainly at the pharmaceutical, agricultural, and energy industries. This technology’s high value is due to its high capacity for the mass production of proteins. Moreover, the ...
Qiao, Lixian;
Jiang, Pingping;
Tang, Yanyan;
Pan, Leilei;
Ji, Hongchang;
Zhou, Wenjie;
Zhu, Hong;
Sui, Jiongming;
Jiang, Defeng;
Wang, Jingshan.
BACKGROUND: Late embryogenesis abundant (LEA) proteins were reported to be related to adversity stress and drought tolerance. Lea-3 from Arachis hypogaea L. (AhLea-3) was previously found to be related to salt tolerance according to the result of transcriptome profiling and digital gene expression analys...
BACKGROUND:
Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant c...
Zea mays/genética,
Cloroplastos/genética,
Cloroplastos/metabolismo,
Proteínas Fluorescentes Verdes/metabolismo,
Nicotiana/metabolismo,
Proteínas Fluorescentes Verdes/genética,
Plastidios/genética,
Plantas Modificadas Genéticamente,
Biotecnología,
Transformación Genética,
Reacción en Cadena de la Polimerasa,
Escherichia coli,
Genoma del Cloroplasto
Abstract we report A. rhizogenes-induced hairy root formation in S. bryopteris, a medicinally and commercially important plant. A. rhizogenes strain LBA1334 co-cultivated with explants (root, rhizophore, stem portion near the root, and stem with intact fronds) for 24 and 48 h after transformation for ind...
We highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independe...
Background: This research is intended to determine suitable types and concentrations of plant growth regulators (PGRs) to induce callus on stem and leaf sections of 4 species of the genus Garcinia, namely, Garcinia mangostana, Garcinia schomburgkiana, Garcinia cowa, and Garcinia celebica. The base medium...
Background: Capsicum is a genus of an important spice crop that belongs to the chili lineage. However, many Capsicum species (family Solanaceae) are known to be recalcitrant to genetic transformation and in vitro regeneration, thus hampering the effort in using Capsicum species for detailed biological in...
Background: Hydrophobins are small proteins secreted by filamentous fungi, which show a highly surface activity. Because of the signally self-assembling abilities and surface activities, hydrophobins were considered as candidates in many aspects, for example, stabilizing foams and emulsions in food produ...
Proteínas Fúngicas/genética,
Proteínas Fúngicas/metabolismo,
Clonación Molecular,
Lentinula/genética,
Lentinula/metabolismo,
Basidiomycota/metabolismo,
Emulsionantes,
Levaduras,
Agrobacterium tumefaciens/metabolismo,
Southern Blotting,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo,
Electroforesis en Gel de Poliacrilamida,
Basidiomycota/enzimología,
Transformación Genética,
Microscopía Fluorescente,
Análisis de Secuencia
Background: The enzymes utilized in the process of beer production are generally sensitive to higher temperatures. About 60% of them are deactivated in drying the malt that limits the utilization of starting material in the fermentation process. Gene transfer from thermophilic bacteria is a promising too...
Thermococcus/metabolismo,
alfa-Amilasas/metabolismo,
Cerveza,
alfa-Amilasas/genética,
Termotolerancia,
Clonación Molecular,
Estabilidad de Enzimas,
Concentración de Iones de Hidrógeno,
Hordeum/enzimología,
Hordeum/genética,
Técnicas de Transferencia de Gen,
Fermentación,
Plantas Modificadas Genéticamente/enzimología,
Transformación Genética,
Semillas/enzimología,
Semillas/genética,
Calor,
Hordeum/microbiología,
Semillas/microbiología
