Results: 14

Cuidado fragmentado: resposta da Rede de Cuidados à Pessoa com Deficiência para crianças com Síndrome Congênita do Zika Vírus

Resumo O objetivo da pesquisa foi analisar a organização e estrutura que a Rede de Cuidados à Pessoa com Deficiência (RCPD) oferece às crianças com Síndrome Congênita do Zika Vírus (SCZV) na Região de Saúde de Salvador (BA). Trata-se de uma pesquisa qualitativa que cotejou Itinerários Terapê...

Analytical and clinical performance of molecular assay used by the Brazilian public laboratory network to detect and discriminate Zika, Dengue and Chikungunya viruses in blood

Braz. j. infect. dis; 25 (2), 2021
ABSTRACT In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered at the Brazilian Regulatory Agency of Health Surveillance - ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their clinical similarities are ...

Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens

Mem. Inst. Oswaldo Cruz; 115 (), 2020
We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From...

Genetic and biological characterisation of Zika virus isolates from different Brazilian regions

Mem. Inst. Oswaldo Cruz; 114 (), 2019
BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the...

Zika detection: comparison of methodologies

Braz. j. microbiol; 49 (1), 2018
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of th...

Simple protocol for population (Sanger) sequencing for Zika virus genomic regions

Mem. Inst. Oswaldo Cruz; 113 (1), 2018
BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE ...

Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols

Braz. j. med. biol. res; 51 (6), 2018
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika dia...

Potential role of dengue virus, chikungunya virus and Zika virus in neurological diseases

Mem. Inst. Oswaldo Cruz; 113 (11), 2018
This study showed that laboratory markers of recent infection by dengue, Zika or chikungunya arboviruses were detected in the biological samples of approximately one-third of patients with encephalitis, myelitis, encephalomyelitis or Guillain-Barré syndrome, in a surveillance programme in Piauí state, ...

Follow up of a robust meta-signature to identify Zika virus infection in Aedes aegypti: another brick in the wall

Mem. Inst. Oswaldo Cruz; 113 (6), 2018
The mosquito Aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. In a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, West Nile and yellow fever viruses. Of these 110 genes, four ...