Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Braz. j. microbiol; 47 (4), 2016
Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2...
ADN Complementario, Virus de la Diarrea Viral Bovina/genética, Genoma Viral, Recombinación Homóloga, Levaduras/genética, Línea Celular, Virus de la Diarrea Viral Bovina/fisiología, Virus de la Diarrea Viral Bovina/ultraestructura, Sistemas de Lectura Abierta, Análisis de Secuencia de ADN, Replicación Viral, Levaduras/metabolismo